PMID- 77723 OWN - NLM STAT- MEDLINE DCOM- 19780814 LR - 20061115 IS - 0008-5472 (Print) IS - 0008-5472 (Linking) VI - 38 IP - 7 DP - 1978 Jul TI - Radioimmunoassays that demonstrate type-specific and group-specific antigenic reactivities for the major internal structural protein of murine mammary tumor viruses. PG - 1990-5 AB - The 28,000-dalton (p28) major structural polypeptide of the mouse mammary tumor virus (MMTV) was isolated and used to develop a highly sensitive and specific radioimmunoassay. Under conditions of limiting antibody in competitive binding assays, as little as 50 pg of purified p28, as well as disrupted MMTV virions and mammary tumor extracts, competed specifically with 125I-labeled MMTV p28. The p28 polypeptide was further shown to contain both group-specific and type-specific antigenic determinants, thus also allowing for further differentiation of various MMTV strains. FAU - Teramoto, Y A AU - Teramoto YA FAU - Schlom, J AU - Schlom J LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Cancer Res JT - Cancer research JID - 2984705R RN - 0 (Antibodies, Viral) RN - 0 (Antigens, Viral) RN - 0 (Epitopes) RN - 0 (Glycoproteins) RN - 0 (Viral Proteins) SB - IM MH - Antibodies, Viral MH - Antigens, Viral/*analysis MH - Binding, Competitive MH - Epitopes MH - Glycoproteins/immunology MH - Mammary Tumor Virus, Mouse/*immunology MH - Molecular Weight MH - Radioimmunoassay/*methods MH - Viral Proteins/*immunology EDAT- 1978/07/01 00:00 MHDA- 1978/07/01 00:01 CRDT- 1978/07/01 00:00 PHST- 1978/07/01 00:00 [pubmed] PHST- 1978/07/01 00:01 [medline] PHST- 1978/07/01 00:00 [entrez] AID - 10.1203/00006450-199501000-00002 [doi] PST - ppublish SO - Cancer Res. 1978 Jul;38(7):1990-5. doi: 10.1203/00006450-199501000-00002.