PMID- 7772668
OWN - NLM
STAT- MEDLINE
DCOM- 19950713
LR  - 20220408
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 16
IP  - 4
DP  - 1995 Mar
TI  - Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for 
      orthopaedic use.
PG  - 287-95
AB  - Using a variety of cell types, cell attachment and growth was studied on 
      prospective (polyethersulphone (PES) and polyetheretherketone) and currently used 
      (titanium 318 alloy, cobalt chrome molybdenum alloy and ultra-high molecular 
      weight polyethylene (UHMWPE)) orthopaedic biomaterials. Proliferation of 
      fibroblasts and osteoblasts was measured using incorporation of tritiated 
      thymidine into total DNA. Attachment of cells was assessed by indirect 
      immunofluorescent labelling of vinculin, a component of the cell's focal adhesion 
      plaque. The degree of cell attachment was quantified on the materials by 
      determining the mean number of adhesion plaques and using an image analysis 
      system to determine the mean total area of plaques per cell. Fibroblasts and 
      osteoblasts responded differently to the materials tested. When grown on PES 
      surfaces, rat tail fibroblasts synthesized significantly greater amounts of DNA 
      than cells on all other surfaces, whilst fibroblasts on UHMWPE synthesized 
      significantly less DNA than cells on all other materials. Interestingly, there 
      was no significant difference between the amounts of DNA synthesized by 
      osteoblasts grown on the various materials. Determination of the number of 
      vinculin adhesion plaques per cell and the mean total area of the plaques per 
      cell showed that the attachment of fibroblasts to UHMWPE was significantly 
      reduced compared with other materials. In contrast there was no significant 
      difference in the adhesion of osteoblasts to different materials. Scanning 
      electron microscope (SEM) observations of cells on the materials correlated with 
      the morphometric data. Cells with the greatest number and area of adhesion 
      plaques were well spread and flattened whilst those with the least number of 
      adhesion plaques were more rounded and less spread.
FAU - Hunter, A
AU  - Hunter A
AD  - Institute of Orthopaedics, University College, Middlesex School of Medicine, 
      University College London, Brockley Hill, Stanmore, Middlesex, UK.
FAU - Archer, C W
AU  - Archer CW
FAU - Walker, P S
AU  - Walker PS
FAU - Blunn, G W
AU  - Blunn GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Actins)
RN  - 0 (Alloys)
RN  - 0 (Benzophenones)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Fluoresceins)
RN  - 0 (Ketones)
RN  - 0 (Polyethylenes)
RN  - 0 (Polymers)
RN  - 0 (Sulfones)
RN  - 25667-42-9 (polyether sulfone)
RN  - 31694-16-3 (polyetheretherketone)
RN  - 3G0H8C9362 (Cobalt)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9007-49-2 (DNA)
RN  - D1JT611TNE (Titanium)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Actins/metabolism
MH  - Alloys
MH  - Analysis of Variance
MH  - Animals
MH  - Benzophenones
MH  - Biocompatible Materials/metabolism
MH  - Bone Neoplasms/pathology
MH  - Cell Adhesion
MH  - Cell Division
MH  - Cells, Cultured
MH  - Cobalt/metabolism
MH  - DNA/biosynthesis
MH  - Fibroblasts/*cytology/metabolism
MH  - Fluorescein
MH  - Fluoresceins/metabolism
MH  - Image Processing, Computer-Assisted
MH  - Ketones/*metabolism
MH  - Microscopy, Electron, Scanning
MH  - Orthopedic Fixation Devices/standards
MH  - Osteoblasts/*cytology/metabolism
MH  - Osteosarcoma/pathology
MH  - Polyethylene Glycols/*metabolism
MH  - Polyethylenes/metabolism
MH  - Polymers/*metabolism
MH  - Rats
MH  - Sulfones/*metabolism
MH  - Titanium/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 014296129593256D [pii]
AID - 10.1016/0142-9612(95)93256-d [doi]
PST - ppublish
SO  - Biomaterials. 1995 Mar;16(4):287-95. doi: 10.1016/0142-9612(95)93256-d.