PMID- 7773557
OWN - NLM
STAT- MEDLINE
DCOM- 19950710
LR  - 20221011
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 4
IP  - 2
DP  - 1995 Mar-Apr
TI  - Repeated intraportal hepatocyte transplantation in analbuminemic rats.
PG  - 237-43
AB  - The optimal site for implantation of isolated hepatocytes has not been 
      established. We have developed a novel technique which allows repeated infusion 
      of hepatocytes into the portal system via an indwelling catheter. Seven Nagase 
      Analbuminemic rats (NAR) underwent single intraportal infusion of 2 x 10(7) 
      isolated normal albumin-producing rat hepatocytes. Another seven NAR rats 
      underwent placement of indwelling catheters into the portal venous system via the 
      gastroduodenal vein. Each of them received six batches of 5 x 10(6) normal 
      albumin producing hepatocytes. Seven control NAR rats were infused repeatedly 
      (intraportally) with saline only. Plasma albumin (ELISA) showed significant 
      increase in experimental animals and was more pronounced (p < 0.05) in rats 
      transplanted repeatedly than in those given a single dose of cells. 
      Immunohistochemical staining of the liver sections confirmed the presence of 
      transplanted albumin producing hepatocytes. Rats transplanted with a single large 
      batch of isolated hepatocytes showed liver tissue damage, whereas those subjected 
      to repeated cell infusions had normal liver histology. We have developed a novel 
      intraportal transplantation method which allows successful engraftment of a large 
      number of isolated hepatocytes.
FAU - Rozga, J
AU  - Rozga J
AD  - Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
FAU - Holzman, M
AU  - Holzman M
FAU - Moscioni, A D
AU  - Moscioni AD
FAU - Fujioka, H
AU  - Fujioka H
FAU - Morsiani, E
AU  - Morsiani E
FAU - Demetriou, A A
AU  - Demetriou AA
LA  - eng
GR  - DL38763-06/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Catheters, Indwelling
MH  - *Cell Transplantation
MH  - Liver/*cytology/metabolism/pathology
MH  - Liver Transplantation/*methods
MH  - Male
MH  - *Portal Vein
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/analysis/*deficiency
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1177/096368979500400207 [doi]
PST - ppublish
SO  - Cell Transplant. 1995 Mar-Apr;4(2):237-43. doi: 10.1177/096368979500400207.