PMID- 7773965
OWN - NLM
STAT- MEDLINE
DCOM- 19950713
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 81
IP  - 1
DP  - 1995 May
TI  - Characterization of a 14q+ marker chromosome in philadelphia chromosome positive 
      acute lymphoblastic leukemia by DNA analysis and fluorescence in situ 
      hybridization.
PG  - 83-91
AB  - We report a case of precursor-B acute lymphoblastic leukemia (ALL) with the 
      Philadelphia chromosome (Ph) and a 14q+ chromosome whose additional material was 
      a part of the long arm of der(9)t(9;22). A minor population carrying the standard 
      Ph translocation without the 14q+ was also observed at the first presentation. 
      The translocation of the BCR gene from chromosome 22 to the subtelomeric region 
      of the 14q+ was confirmed by fluorescence in situ hybridization (FISH) using a 
      yeast artificial chromosome (YAC) clone containing the BCR gene. The breakpoint 
      of chromosome 14 could not be determined exactly but probably was at 14q24 or 
      14q32 by conventional chromosome analysis. Nevertheless, FISH using a YAC clone 
      containing the human immunoglobulin heavy chain (IgH) gene locus, Southern blot, 
      and pulsed-field gel electrophoresis (PFGE) analyses with IgJH probe, and loss of 
      heterozygosity analysis at the alpha 1-antitrypsin (AT) gene locus showed lack of 
      involvement of the IgH gene in the 14q+ and more centromeric breakage than the 
      alpha 1-AT locus at 14q32.1. Thus, the formation of the 14q+ seemed to be a 
      secondary genetic event after the Ph translocation and presumably played a minor 
      role in the pathogenesis of B-cell malignancy in this case.
FAU - Nakai, H
AU  - Nakai H
AD  - Third Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Tanaka, S
AU  - Tanaka S
FAU - Nishigaki, H
AU  - Nishigaki H
FAU - Nakagawa, H
AU  - Nakagawa H
FAU - Misawa, S
AU  - Misawa S
FAU - Kashima, K
AU  - Kashima K
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (alpha 1-Antitrypsin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (BCR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
MH  - Base Sequence
MH  - Bone Marrow/ultrastructure
MH  - *Chromosomes, Human, Pair 14
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Genes, Immunoglobulin/genetics
MH  - Genes, abl/genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Oncogene Proteins/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - *Protein-Tyrosine Kinases
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-bcr
MH  - Translocation, Genetic
MH  - alpha 1-Antitrypsin/genetics
RF  - 33
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - S0165460894002061 [pii]
AID - 10.1016/s0165-4608(94)00206-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 May;81(1):83-91. doi: 10.1016/s0165-4608(94)00206-1.