PMID- 7775381
OWN - NLM
STAT- MEDLINE
DCOM- 19950707
LR  - 20190512
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 117
IP  - 1
DP  - 1995 Jan
TI  - Cloning and characterization of cDNA for inter-alpha-trypsin inhibitor family 
      heavy chain-related protein (IHRP), a novel human plasma glycoprotein.
PG  - 14-8
AB  - The cDNA encoding inter-alpha-trypsin inhibitor family heavy chain-related 
      protein (IHRP) was cloned from human liver cDNA libraries. Oligonucleotide 
      primers of human liver cDNA for PCR were constructed from internal amino acid 
      sequences obtained with proteolytic fragments of IHRP. The amplified cDNA served 
      as a hybridization probe for the screening of human liver cDNA libraries. The 
      cDNA of 2,977 bp contained an entire reading frame coding 930 amino acids. The 
      N-terminal 28 residues corresponded to a signal peptide for secretion. The 
      N-terminal 600 residues of the mature form exhibited considerable homology to 
      those of ITI heavy chains, while the C-terminal 300 residues showed no homology 
      with the heavy chains and low homology with ATP-dependent proteases. IHRP was 
      readily cleaved into 85- and 35-kDa fragments when plasma was incubated at 37 
      degrees C. The cleaved site, Arg-Arg-Leu, was within a proline-rich region. 
      Northern blot analysis of poly(A) RNAs from various human tissues only showed 
      hybridization to liver RNA.
FAU - Saguchi, K
AU  - Saguchi K
AD  - Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa 
      University, Tokyo.
FAU - Tobe, T
AU  - Tobe T
FAU - Hashimoto, K
AU  - Hashimoto K
FAU - Sano, Y
AU  - Sano Y
FAU - Nakano, Y
AU  - Nakano Y
FAU - Miura, N H
AU  - Miura NH
FAU - Tomita, M
AU  - Tomita M
LA  - eng
SI  - GENBANK/D38595
PT  - Journal Article
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Blood Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycoproteins)
RN  - 0 (ITIH4 protein, human)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blood Proteins/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Gene Library
MH  - Glycoproteins/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a124701 [doi]
PST - ppublish
SO  - J Biochem. 1995 Jan;117(1):14-8. doi: 10.1093/oxfordjournals.jbchem.a124701.