PMID- 7775584
OWN - NLM
STAT- MEDLINE
DCOM- 19950707
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 129
IP  - 5
DP  - 1995 Jun
TI  - Hepatocyte growth factor/scatter factor modulates cell motility, proliferation, 
      and proteoglycan synthesis of chondrocytes.
PG  - 1411-9
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional growth 
      factor that promotes proliferation, motility, and morphogenesis in epithelial 
      cells. Recently the HGF receptor, c-met protooncogene product, has been shown to 
      be expressed in developing limb buds (Sonnenberg, E., D. Meyer, M. Weidner, and 
      C. Birchmeiyer, 1993. J. Cell Biol. 123: 223-235), suggesting that some 
      populations of mesenchymal cells in limb buds respond to HGF/SF. To test the 
      possibility that HGF/SF is involved in regulation of cartilage development, we 
      isolated chondrocytes from knee joints and costal cartilages of 23-d embryonic 
      and 4-wk-old rabbits, and analyzed the effects of HGF/SF on migration and 
      proliferation of these cells. We found that HGF/SF stimulated migration of 
      cultured articular chondrocytes but did not scatter limb mesenchymal fibroblasts 
      or synovial fibroblasts in culture. HGF/SF also stimulated proliferation of 
      chondrocytes; a maximum three-fold stimulation in DNA synthesis was observed at 
      the concentration of 3 ng/ml of HGF/SF. Moreover, HGF/SF had the ability to 
      enhance proteoglycan synthesis in chondrocytes. The responsiveness of 
      chondrocytes to HGF/SF was also supported by the observation that they expressed 
      the HGF/SF receptor. Addition of the neutralizing antibody to rat HGF/SF affected 
      neither DNA synthesis nor proteoglycan synthesis in rat chondrocytes, suggesting 
      a paracine mechanism of action of HGF/SF on these cells. In situ hybridization 
      analysis showed that HGF/SF mRNA was restrictively expressed in the areas of 
      future joint regions in developing limb buds and in the intercostal spaces of 
      developing costal cartilages. These findings suggest that HGF/SF plays important 
      roles in cartilage development through its multiple activities.
FAU - Takebayashi, T
AU  - Takebayashi T
AD  - Department of Oral Surgery, Okayama University Dental School, Japan.
FAU - Iwamoto, M
AU  - Iwamoto M
FAU - Jikko, A
AU  - Jikko A
FAU - Matsumura, T
AU  - Matsumura T
FAU - Enomoto-Iwamoto, M
AU  - Enomoto-Iwamoto M
FAU - Myoukai, F
AU  - Myoukai F
FAU - Koyama, E
AU  - Koyama E
FAU - Yamaai, T
AU  - Yamaai T
FAU - Matsumoto, K
AU  - Matsumoto K
FAU - Nakamura, T
AU  - Nakamura T
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Cartilage/cytology/*drug effects/embryology
MH  - Cell Division/drug effects
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Embryonic and Fetal Development
MH  - Hepatocyte Growth Factor/biosynthesis/*pharmacology
MH  - Proteoglycans/*biosynthesis
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Rats
MH  - Rats, Wistar
PMC - PMC2120469
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
PMCR- 1995/12/01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
PHST- 1995/12/01 00:00 [pmc-release]
AID - 95294142 [pii]
AID - 10.1083/jcb.129.5.1411 [doi]
PST - ppublish
SO  - J Cell Biol. 1995 Jun;129(5):1411-9. doi: 10.1083/jcb.129.5.1411.