PMID- 7775586
OWN - NLM
STAT- MEDLINE
DCOM- 19950711
LR  - 20131121
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 163
IP  - 3
DP  - 1995 Jun
TI  - Patterns of cyclic AMP-dependent protein kinase gene expression during ontogeny 
      of the murine palate.
PG  - 431-40
AB  - Normal growth and differentiation of embryonic palatal tissue depends on 
      regulated levels of intracellular cAMP. Cyclic AMP-dependent protein kinases 
      (PKA) act to mediate the biological activities of cAMP. PKA isozyme protein 
      profiles demonstrate a clear pattern of temporal alterations in embryonic palatal 
      tissue during its development. In order to ascertain the molecular basis for 
      changing PKA isozyme profiles during palatal ontogeny, the spatial and temporal 
      expression of mRNAs for regulatory (RI alpha, RII alpha, and RII beta) and 
      catalytic (C alpha) subunits of PKA was examined. RNA extracted from murine 
      embryonic palatal tissue (days 12-14 of gestation) was examined by Northern blot 
      analysis. Significant levels of constitutively expressed RI alpha and C alpha 
      mRNA were seen on all days of gestation examined. RI alpha transcripts were 
      substantially less abundant in palate mesenchymal cells in vitro than in palatal 
      tissue in vivo. Levels of RII alpha and RII beta mRNA were highest on gestational 
      day (GD) 12, a period characterized by pronounced palatal tissue growth. In 
      addition, patterns of tissue distribution of RII beta, not previously described, 
      were examined in the developing embryonic palate. A dramatic developmental shift 
      in tissue distribution of RII beta was seen. The isozyme was evenly distributed 
      between palatal epithelial and mesenchymal cells on GD 12 but by GD 14, RII beta 
      was predominantly localized to palatal epithelial cells. Direct activation of 
      adenylate cyclase with forskolin in murine embryonic palate mesenchymal (MEPM) 
      cells resulted in an increase in RII alpha mRNA levels but had no effect on 
      steady state levels of RII beta or C alpha mRNA. In addition, elevation of 
      intracellular levels of cAMP resulted in a shift in the transcriptional profile 
      of RI alpha mRNAs. Results of this study document specific patterns of expression 
      for the genes encoding the various cAMP-dependent protein kinase regulatory and C 
      alpha subunits in murine embryonic palatal tissue. In addition, we have 
      demonstrated adaptational changes of this kinase in MEPM cells in response to 
      conditions of increased intracellular levels of cAMP.
FAU - Greene, R M
AU  - Greene RM
AD  - Department of Pathology, Anatomy, and Cell Biology, Jefferson Medical College, 
      Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
FAU - Lloyd, M R
AU  - Lloyd MR
FAU - Uberti, M
AU  - Uberti M
FAU - Nugent, P
AU  - Nugent P
FAU - Pisano, M M
AU  - Pisano MM
LA  - eng
GR  - DE-05550/DE/NIDCR NIH HHS/United States
GR  - DE-08199/DE/NIDCR NIH HHS/United States
GR  - DE10323/DE/NIDCR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit)
RN  - 0 (Cyclic AMP-Dependent Protein Kinase RIalpha Subunit)
RN  - 0 (Cyclic AMP-Dependent Protein Kinase RIbeta Subunit)
RN  - 0 (Prkar1a protein, mouse)
RN  - 0 (Prkar1b protein, mouse)
RN  - 0 (Prkar2a protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit
MH  - Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
MH  - Cyclic AMP-Dependent Protein Kinase RIbeta Subunit
MH  - Cyclic AMP-Dependent Protein Kinases/*genetics
MH  - Female
MH  - Fetus/*physiology
MH  - *Gene Expression
MH  - Immunohistochemistry
MH  - Intracellular Membranes/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Palate/*embryology
MH  - RNA, Messenger/metabolism
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1002/jcp.1041630302 [doi]
PST - ppublish
SO  - J Cell Physiol. 1995 Jun;163(3):431-40. doi: 10.1002/jcp.1041630302.