PMID- 7776241
OWN - NLM
STAT- MEDLINE
DCOM- 19950712
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 483 ( Pt 1)
IP  - Pt 1
DP  - 1995 Feb 15
TI  - Immunoglobulin E receptor-activated calcium conductance in rat mast cells.
PG  - 59-66
AB  - 1. The nystatin perforated-patch method was used to record macroscopic currents 
      from anti-trinitrophenyl (TNP) immunoglobulin E (IgE)-sensitized rat basophilic 
      leukaemia (RBL-2H3) cells at 37 degrees C. 2. An inwardly rectifying Ca2+ current 
      (ICa) was activated upon stimulation with the multivalent antigen 
      trinitrophenylated bovine serum albumin (TNP-BSA). Induction of ICa was not 
      observed at room temperature. ICa was reversed and reinduced upon cyclical 
      addition of the monovalent hapten dinitrophenyl (DNP)-lysine and multivalent 
      antigen, indicating that a specific interaction of antigen with IgE was required 
      to elicit ICa. 3. The antigen-induced current was also carried by Ba2+ or Sr2+, 
      and to a lesser extent by Na+, in the nominal absence of Ca2+. ICa did not 
      exhibit time-dependent opening (< or = 1 ms) in response to hyperpolarizing 
      voltage steps to -100 mV, although it did accumulate steady-state inactivation of 
      approximately 40-50% over 100 ms. 4. Two inorganic blockers of antigen-stimulated 
      45Ca2+ influx and secretion, La3+ and Zn2+, inhibited ICa by approximately 50% at 
      concentrations known to produce 50% block of 45Ca2+ influx. In contrast, cromolyn 
      sodium (0.5 mM) and the L-type Ca2+ channel antagonist nitrendipine (5 microM) 
      had no effect on ICa. 5. ICa also was induced by the intracellular Ca2+ mobilizer 
      thapsigargin. Because the actions of thapsigargin and antigen were not additive, 
      IgE receptor cross-linkage appears to activate the recently described 
      capacitative Ca2+ entry channels.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Zoology and Genetics, Iowa State University, Ames 50011-3223, USA.
FAU - McCloskey, M A
AU  - McCloskey MA
LA  - eng
GR  - GM48144/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Antigens)
RN  - 0 (Calcium Channels)
RN  - 0 (Haptens)
RN  - 0 (Receptors, IgE)
RN  - 0 (Terpenes)
RN  - 24GP945V5T (Barium)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6I3K30563S (Lanthanum)
RN  - 9B627AW319 (Nitrendipine)
RN  - 9NEZ333N27 (Sodium)
RN  - Q2WXR1I0PK (Cromolyn Sodium)
RN  - SY7Q814VUP (Calcium)
RN  - YZS2RPE8LE (Strontium)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Barium/metabolism
MH  - Calcium/*metabolism
MH  - Calcium Channels/physiology
MH  - Cromolyn Sodium/pharmacology
MH  - Haptens/immunology
MH  - Immunoglobulin E/immunology/*metabolism
MH  - Lanthanum/pharmacology
MH  - Mast Cells/*metabolism
MH  - Membrane Potentials/physiology
MH  - Nitrendipine/pharmacology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Receptors, IgE/*metabolism
MH  - Sodium/metabolism
MH  - Strontium/metabolism
MH  - Terpenes/pharmacology
MH  - Thapsigargin
MH  - Tumor Cells, Cultured
PMC - PMC1157871
EDAT- 1995/02/15 00:00
MHDA- 1995/02/15 00:01
PMCR- 1995/02/15
CRDT- 1995/02/15 00:00
PHST- 1995/02/15 00:00 [pubmed]
PHST- 1995/02/15 00:01 [medline]
PHST- 1995/02/15 00:00 [entrez]
PHST- 1995/02/15 00:00 [pmc-release]
AID - 10.1113/jphysiol.1995.sp020567 [doi]
PST - ppublish
SO  - J Physiol. 1995 Feb 15;483 ( Pt 1)(Pt 1):59-66. doi: 
      10.1113/jphysiol.1995.sp020567.