PMID- 7779576
OWN - NLM
STAT- MEDLINE
DCOM- 19950714
LR  - 20181113
IS  - 0163-4984 (Print)
IS  - 0163-4984 (Linking)
VI  - 47
IP  - 1-3
DP  - 1995 Jan-Mar
TI  - Effect of aluminum and lead salts on lipid peroxidation and cell survival in 
      human skin fibroblasts.
PG  - 57-67
AB  - The aim of this study was to see whether aluminum (Al) and lead (Pb) salts are 
      toxic for cultured human fibroblasts under different experimental conditions, in 
      the controllable situation offered by cell cultures. Cell survival and membrane 
      lipid peroxidation served as markers of Al and Pb toxicity. Evaluation of the 
      living cells was carried out using a colorimetric method, the mitochondrial 
      reduction of 1-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). 
      Lipoperoxidation assay was performed on whole cell homogenates by measuring 
      thiobarbituric acid-reactive substances (TBARS) produced after incubation with 
      ascorbic acid-ferrous sulfate. Al(III) and Pb(II) salts (300 microM) produce a 
      considerable decrease in cell survival after an exposure period of 4d, evident 
      with the three fetal calf serum concentrations in the culture media: 2, 5, and 
      10%. Taking into account in vitro cell aging, the cytotoxic effects of Al(III) 
      and Pb(II) are greater in senescent fibroblasts than in young cells. Lead-induced 
      cytotoxicity is higher than Al-induced cytotoxicity. A mechanism that contributes 
      to cellular toxicity is membrane lipid peroxidation; our results demonstrate that 
      Al(III) and Pb(II) ions, 400 microM, exert an antioxidant-like effect or a 
      pro-oxidant action on cell membranes depending on exposure time. We describe 
      significant increases in TBARS formation associated with the presence of 400 
      microM Al(III) or Pb(II) salts in the culture media. Our study also revealed that 
      these heavy metals induce a cell age-dependent action on membrane 
      lipoperoxidation that is greater in senescent fibroblasts and this could have 
      severe consequences for maintenance of cellular integrity.
FAU - Dominguez, M C
AU  - Dominguez MC
AD  - Unitat de Recerca Biomedica, Hospital Universitari Materno-infantil Vall 
      d'Hebron, Barcelona, Spain.
FAU - Sole, E
AU  - Sole E
FAU - Goni, C
AU  - Goni C
FAU - Ballabriga, A
AU  - Ballabriga A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Aluminum Compounds)
RN  - 0 (Ferrous Compounds)
RN  - 0 (Nitrates)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 2P299V784P (Lead)
RN  - 39R4TAN1VT (ferrous sulfate)
RN  - 4IL61GN3YI (lead chloride)
RN  - HUO854648Y (aluminum nitrate)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Aluminum Compounds/*pharmacology
MH  - Ascorbic Acid/pharmacology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Ferrous Compounds/pharmacology
MH  - Fibroblasts/cytology/drug effects/metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Kinetics
MH  - Lead/*pharmacology
MH  - Lipid Peroxidation/*drug effects
MH  - Nitrates/*pharmacology
MH  - Skin/*cytology/*metabolism
MH  - Thiobarbituric Acid Reactive Substances/analysis
MH  - Time Factors
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF02790101 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 1995 Jan-Mar;47(1-3):57-67. doi: 10.1007/BF02790101.