PMID- 7779996
OWN - NLM
STAT- MEDLINE
DCOM- 19950720
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 52
IP  - 4
DP  - 1995 Apr
TI  - Tumor necrosis factor alpha inhibits rat granulosa cell plasminogen activator 
      activity in vitro during follicular development.
PG  - 745-52
AB  - The objective of the present in vitro study was to examine the potential 
      modulatory influence of tumor necrosis factor-alpha (TNF alpha) on the granulosa 
      cell plasminogen activator (PA) system during follicular development. 
      Undifferentiated and differentiated rat granulosa cells of preantral follicles 
      and antral follicles, respectively, were cultured in a chemically defined medium 
      with or without TNF alpha and in the absence or presence of FSH (400 ng/ml). TNF 
      alpha (0.5-50 ng/ml) inhibited basal and FSH-induced net PA activities in 
      cultures of granulosa cells from preantral and antral follicles in a 
      concentration- and time-dependent manner. Although PA activities with 
      corresponding molecular masses of 55 kDa and 30 kDa (tissue-[tPA] and 
      urokinase-[uPA] type PA, respectively) were observed in culture of 
      undifferentiated granulosa cells, only tPA was detectable in differentiated 
      cells. Concomitant to the stimulation in PA activities by FSH was a marked 
      increase in progestin secretion and a decrease in DNA synthetic capacity at both 
      stages of follicular development. Independent of the differentiative state of the 
      granulosa cells, TNF alpha suppressed FSH-stimulated tPA activity, but 
      potentiated FSH-induced uPA activity in undifferentiated granulosa cells. The 
      inhibition of the gonadotropin action by TNF alpha was accompanied by an increase 
      in PA inhibitor activity, which was more pronounced in cultures of differentiated 
      granulosa cells. TNF alpha inhibited FSH-induced progestin secretion and reversed 
      the action of the gonadotropin on DNA synthesis irrespective of stage of 
      follicular maturation. These studies demonstrate that TNF alpha modulates 
      gonadotropic action on granulosa cell differentiation (PA and progestin 
      secretion) and proliferation (DNA synthesis) during follicular 
      development.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Karakji, E G
AU  - Karakji EG
AD  - Department of Obstetrics & Gynaecology, University of Ottawa, Ontario, Canada.
FAU - Tsang, B K
AU  - Tsang BK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Culture Media)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Culture Media
MH  - Diethylstilbestrol/pharmacology
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Granulosa Cells/*enzymology
MH  - Kinetics
MH  - Male
MH  - Molecular Weight
MH  - Ovarian Follicle/*physiology
MH  - Progesterone/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Plasminogen Activator/*antagonists & inhibitors/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Urokinase-Type Plasminogen Activator/*antagonists & inhibitors/metabolism
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1095/biolreprod52.4.745 [doi]
PST - ppublish
SO  - Biol Reprod. 1995 Apr;52(4):745-52. doi: 10.1095/biolreprod52.4.745.