PMID- 7782072 OWN - NLM STAT- MEDLINE DCOM- 19950720 LR - 20190904 IS - 0888-7543 (Print) IS - 0888-7543 (Linking) VI - 26 IP - 1 DP - 1995 Mar 1 TI - A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis. PG - 134-7 AB - The physical locations of 46 cosmid clones and 21 P1 clones were determined along the chromosome 20 axis relative to the p terminus (FLpter) using fluorescence in situ hybridization (FISH) and digital image microscopy. The cosmid clones were selected from the chromosomally enriched library LA20NC01. Nine P1 clones were selected from a pooled DuPont genomic library using PCR with primer pairs selected to amplify genetically mapped sequence-tagged sites. This information was used to relate the physical map to the genetic map. Twelve P1 clones were selected from the same library using PCR primer pairs that amplified known genes. Two of these, E2F and BCLX, had not been mapped previously. FAU - Stokke, T AU - Stokke T AD - University of California, San Francisco, USA. FAU - Collins, C AU - Collins C FAU - Kuo, W L AU - Kuo WL FAU - Kowbel, D AU - Kowbel D FAU - Shadravan, F AU - Shadravan F FAU - Tanner, M AU - Tanner M FAU - Kallioniemi, A AU - Kallioniemi A FAU - Kallioniemi, O P AU - Kallioniemi OP FAU - Pinkel, D AU - Pinkel D FAU - Deaven, L AU - Deaven L AU - et al. LA - eng GR - CA 58207/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Genomics JT - Genomics JID - 8800135 SB - IM MH - Base Sequence MH - Chromosome Mapping/*methods MH - Chromosomes, Human, Pair 20/*genetics MH - Genetic Linkage MH - Genomic Library MH - Humans MH - *Image Processing, Computer-Assisted MH - *In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Polymerase Chain Reaction MH - Sequence Tagged Sites EDAT- 1995/03/01 00:00 MHDA- 1995/03/01 00:01 CRDT- 1995/03/01 00:00 PHST- 1995/03/01 00:00 [pubmed] PHST- 1995/03/01 00:01 [medline] PHST- 1995/03/01 00:00 [entrez] AID - 10.1016/0888-7543(95)80092-z [doi] PST - ppublish SO - Genomics. 1995 Mar 1;26(1):134-7. doi: 10.1016/0888-7543(95)80092-z.