PMID- 7782073
OWN - NLM
STAT- MEDLINE
DCOM- 19950720
LR  - 20190904
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 26
IP  - 1
DP  - 1995 Mar 1
TI  - Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and 
      polymorphic markers.
PG  - 138-41
AB  - We report the chromosomal localization and a simple sequence repeat (SSR) in the 
      cytosolic phospholipase A2 (cPLA2) gene in both human and rat. A (CA)18 repeat in 
      the promoter of the rat gene was determined to exhibit length polymorphism when 
      analyzed using the polymerase chain reaction (PCR) in 19 different inbred rat 
      strains. Genotyping for this marker in 234 F2 progeny of a SHR x BN intercross 
      mapped the gene to rat chromosome 13. Using a PCR strategy, a fragment of the 
      promoter for the human gene was isolated, and a (CA)18 repeat was identified. 
      Since this marker displayed a low heterozygosity index, we also identified a 
      mononucleotide repeat in the promoter for cPLA2 that displayed a polymorphism 
      information content value of 0.76. The human gene was mapped using fluorescence 
      in situ hybridization (FISH) to chromosome 1q25. Of interest, the gene encoding 
      the enzyme prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2), which acts 
      on the arachidonic acid product of cPLA2, was previously localized to this same 
      chromosomal region, raising the possibility of coordinate regulation. 
      Identification of intragenic markers may facilitate studies of polymorphic 
      variants of these genes as candidates for disorders in which perturbations of the 
      eicosanoid cascade may play a role.
FAU - Tay, A
AU  - Tay A
AD  - Membrane Biology Group, Hospital for Sick Children, Toronto, Canada.
FAU - Simon, J S
AU  - Simon JS
FAU - Squire, J
AU  - Squire J
FAU - Hamel, K
AU  - Hamel K
FAU - Jacob, H J
AU  - Jacob HJ
FAU - Skorecki, K
AU  - Skorecki K
LA  - eng
SI  - GENBANK/U08374
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
GS  - cPLA2
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Crosses, Genetic
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Phospholipases A/*genetics
MH  - Phospholipases A2
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 0888-7543(95)80093-2 [pii]
AID - 10.1016/0888-7543(95)80093-2 [doi]
PST - ppublish
SO  - Genomics. 1995 Mar 1;26(1):138-41. doi: 10.1016/0888-7543(95)80093-2.