PMID- 7782765
OWN - NLM
STAT- MEDLINE
DCOM- 19950720
LR  - 20161123
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 76 ( Pt 6)
DP  - 1995 Jun
TI  - Production, characterization and reactivity of monoclonal antibodies to porcine 
      reproductive and respiratory syndrome virus.
PG  - 1361-9
AB  - This report describes the preparation of six monoclonal antibodies (MAbs) raised 
      against a British isolate of porcine reproductive and respiratory syndrome virus 
      (PRRSV), their characterization in terms of protein specificity and their 
      reactivity with different PRRS viruses from Europe and the USA. 
      Radioimmunoprecipitation and Western blotting studies of MAb reactivity with 
      proteins from cell lysates of infected cells and purified virus revealed that 
      four of the six MAbs (WBE1 and WBE4-6) precipitated a 15 kDa viral protein. 
      Further studies using in vitro translated products of the Lelystad virus genome 
      showed that this protein was the product of ORF7, the putative nucleocapsid 
      protein. The specificity of another MAb, WBE2, was found to be for a 45 kDa 
      protein, determined to be the product of ORF3 and demonstrated to be present in 
      purified virion preparations. The protein specificity of the sixth MAb, WBE3 
      could not be determined. Thirty-three PRRSV isolates from Europe and the USA were 
      grown in alveolar macrophages and examined by immunoperoxidase staining, using 
      the panel of six MAbs. All European isolates were recognized by the four MAbs 
      specific for the putative nucleocapsid, but the viruses showed different patterns 
      of reactivity with WBE2 and WBE3. Furthermore, these two MAbs stained only a 
      small proportion of the cells infected with certain isolates, suggesting that a 
      single isolate may be antigenically heterogeneous. No MAbs bound to US isolates, 
      indicating a consistent antigenic difference between the putative nucleocapsid of 
      US and European isolates. Detergent extraction of cell lysate antigen abrogated 
      the binding of WBE1-3, suggesting that the epitopes are conformation dependent.
FAU - Drew, T W
AU  - Drew TW
AD  - CVL (Weybridge), New Haw, Addlestone, UK.
FAU - Meulenberg, J J
AU  - Meulenberg JJ
FAU - Sands, J J
AU  - Sands JJ
FAU - Paton, D J
AU  - Paton DJ
LA  - eng
SI  - GENBANK/L77913
SI  - GENBANK/L77914
SI  - GENBANK/L77915
SI  - GENBANK/L77916
SI  - GENBANK/L77917
SI  - GENBANK/L77918
SI  - GENBANK/L77919
SI  - GENBANK/L77920
SI  - GENBANK/L77921
SI  - GENBANK/L77922
SI  - GENBANK/L77923
SI  - GENBANK/L77924
SI  - GENBANK/L77925
SI  - GENBANK/L77926
SI  - GENBANK/L77927
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Primers)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal/biosynthesis
MH  - Arterivirus/genetics/immunology/*isolation & purification
MH  - Arterivirus Infections/*veterinary/virology
MH  - Base Sequence
MH  - Blotting, Western
MH  - DNA Primers
MH  - Europe
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - Swine
MH  - Swine Diseases/*virology
MH  - United Kingdom
MH  - United States
MH  - Viral Proteins/analysis/biosynthesis
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1099/0022-1317-76-6-1361 [doi]
PST - ppublish
SO  - J Gen Virol. 1995 Jun;76 ( Pt 6):1361-9. doi: 10.1099/0022-1317-76-6-1361.