PMID- 7787436
OWN - NLM
STAT- MEDLINE
DCOM- 19950727
LR  - 20131121
IS  - 1050-7256 (Print)
IS  - 1050-7256 (Linking)
VI  - 5
IP  - 1
DP  - 1995 Feb
TI  - Human liver triiodothyronine sulfotransferase: copurification with phenol 
      sulfotransferases.
PG  - 61-6
AB  - To ascertain whether triiodothyronine (T3) sulfotransferase coeluted with the 
      known phenol sulfotransferases (PSTs) during purification, human liver 
      thermostable PST, thermolabile PST, and T3 sulfotransferase were assayed with 
      p-nitrophenol, dopamine, and T3, respectively. Thermostable PST eluted from an 
      ion-exchange column in two sequential peaks of activity (Peaks I and II), 
      followed by a peak of thermolabile PST activity. There were three peaks of T3 
      sulfotransferase with thermostable PST: two within thermostable PST Peak I, and 
      one peak of T3 sulfotransferase activity within thermostable PST Peak II. There 
      was a minor peak of T3 sulfotransferase with thermolabile PST. Further 
      purification of thermostable Peak I showed coelution of T3 sulfotransferase with 
      thermostable PST during gel filtration and affinity chromatography. SDS-PAGE 
      revealed a major protein band at 31 kDa. Dehydroepiandrosterone sulfotransferase 
      comprised only 4% of the final activity. This report demonstrates coelution of T3 
      sulfotransferase with thermostable PST, shows a potential additional isozyme of 
      T3 sulfotransferase, and points out the apparent minimal role of 
      dehydroepiandrosterone sulfotransferase in T3 sulfation. The findings support the 
      hypothesis that thermostable PST is the predominant human liver T3 
      sulfotransferase activity.
FAU - Anderson, R J
AU  - Anderson RJ
AD  - Department of Medicine, Department of Veterans Affairs Medical Center, Omaha, 
      Nebraska, USA.
FAU - Babbitt, L L
AU  - Babbitt LL
FAU - Liebentritt, D K
AU  - Liebentritt DK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thyroid
JT  - Thyroid : official journal of the American Thyroid Association
JID - 9104317
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.- (dehydroepiandrosterone sulfotransferase)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Arylsulfotransferase/*isolation & purification
MH  - Child
MH  - Chromatography, Agarose
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Liver/*enzymology
MH  - Male
MH  - Sulfotransferases/isolation & purification
MH  - Triiodothyronine/*metabolism
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1089/thy.1995.5.61 [doi]
PST - ppublish
SO  - Thyroid. 1995 Feb;5(1):61-6. doi: 10.1089/thy.1995.5.61.