PMID- 7787766
OWN - NLM
STAT- MEDLINE
DCOM- 19950725
LR  - 20190830
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 26
IP  - 1
DP  - 1995 Jan
TI  - Rapid and gentle extraction, reconstitution and characterization of microfilament 
      and glia filament from rat astrocytes.
PG  - 91-7
AB  - We developed gentle and rapid methods for depolymerization and extraction of both 
      microfilament and glia filament separately from a crude cytoskeletal fraction of 
      rat astrocytes. Electron microscopy revealed that the filament reconstituted from 
      the microfilament extract closely resembled F-actin that was formed from G-actin 
      of rabbit skeletal muscle. It was found by immunoblotting analysis that even the 
      reconstituted microfilament-like filaments, which had been purified by affinity 
      chromatography with heavy meromyosin subfragment 1 (S1)-conjugated Sepharose, 
      contained vimentin and glia fibrillary acidic protein (GFAP) besides actin, 
      inferring the interaction between microfilament and glia filament. The filaments 
      (9-10 nm thick) reconstituted from the glia filament extract were composed of 
      actin and other minor components in addition to vimentin and GFAP. Actin, GFAP, 
      101, 34, 32.5, 30.5, 29.5 and 28 kDa proteins found in the reconstituted glia 
      filament-like filaments were suggested to be glia filament-associated proteins.
FAU - Ito, J
AU  - Ito J
AD  - Department of Biochemistry, Nagoya City University Medical School, Japan.
FAU - Masuda, M
AU  - Masuda M
FAU - Tada, T
AU  - Tada T
FAU - Tanaka, R
AU  - Tanaka R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Biopolymers)
RN  - 02F3473H9O (Magnesium Chloride)
RN  - 660YQ98I10 (Potassium Chloride)
SB  - IM
MH  - *Actin Cytoskeleton/chemistry/drug effects/ultrastructure
MH  - Animals
MH  - Astrocytes/chemistry/*ultrastructure
MH  - Biopolymers
MH  - Cell Fractionation/methods
MH  - *Intermediate Filaments/chemistry/drug effects/ultrastructure
MH  - Magnesium Chloride/pharmacology
MH  - Microscopy, Electron
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 0197018694000848 [pii]
AID - 10.1016/0197-0186(94)00084-8 [doi]
PST - ppublish
SO  - Neurochem Int. 1995 Jan;26(1):91-7. doi: 10.1016/0197-0186(94)00084-8.