PMID- 7787882
OWN - NLM
STAT- MEDLINE
DCOM- 19950725
LR  - 20131121
IS  - 0968-8773 (Print)
IS  - 0968-8773 (Linking)
VI  - 40
IP  - 7-8
DP  - 1994
TI  - In vivo binding of proteins to stably integrated MMTV DNA in murine cell lines: 
      occupancy of NFI and OTF1 binding sites in the absence and presence of 
      glucocorticoids.
PG  - 643-52
AB  - Activation of expression at the mouse mammary tumor virus (MMTV) promoter is 
      thought to be controlled by nucleosome positioning. On stably integrated MMTV 
      DNA, the long terminal repeat (LTR) region is organized in a phased array of 
      nucleosomes which allegedly occludes transcription factors such as NFI from 
      binding. NFI only binds to the promoter region when the ordered nucleosome 
      structure is apparently disrupted by activated steroid hormone receptors in 
      hormone induced transcription. In certain cell lines, binding sites for the 
      transcription factors NFI and OTF1 are however required for hormone-independent 
      expression of MMTV. We have used stably transfected mouse NIH3T3 and GR cells 
      that exhibit detectable MMTV expression in the absence of hormone for in vivo 
      determination of proteins binding to the MMTV promoter. Here, we present in vivo 
      dimethyl sulfate footprinting data that show that the NFI and OTF binding sites 
      are permanently occupied in vivo in these cells. The contacting guanine residues 
      identified in vivo were demonstrated in in vitro methylation interference assays 
      to correspond to binding by NFI and OTF1. These results demonstrate a novel 
      feature of transcription factor occupancy at the MMTV LTR promoter.
FAU - Hartig, E
AU  - Hartig E
AD  - Kernforschungszentrum Karlsruhe, Institute of Genetics, Germany.
FAU - Cato, A C
AU  - Cato AC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Mol Biol Res
JT  - Cellular & molecular biology research
JID - 9316986
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hcfc1 protein, mouse)
RN  - 0 (Host Cell Factor C1)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (Octamer Transcription Factor-1)
RN  - 0 (Pou2f1 protein, mouse)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (transcription factor nuclear factor 1)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis/metabolism
MH  - DNA Primers
MH  - DNA, Viral/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation, Viral
MH  - Host Cell Factor C1
MH  - Mammary Tumor Virus, Mouse/*genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nuclear Proteins/metabolism
MH  - Nucleosomes/metabolism
MH  - Octamer Transcription Factor-1
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/biosynthesis/*metabolism
MH  - Recombinant Proteins/biosynthesis/metabolism
MH  - Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Sequence Deletion
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic/drug effects
MH  - Transfection
MH  - *Virus Integration
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol Res. 1994;40(7-8):643-52.