PMID- 7787993
OWN - NLM
STAT- MEDLINE
DCOM- 19950727
LR  - 20190913
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 45
IP  - 3
DP  - 1995 Mar
TI  - Interphase cytogenetics of gastric carcinoma: fluorescence in situ hybridization 
      (FISH) applied to cells obtained from formalin-fixed paraffin-embedded tissues.
PG  - 227-32
AB  - The interphase cytogenetics in formalin-fixed and paraffin-embedded gastric 
      cancer tissues were examined by fluorescence in situ hybridization (FISH) with 
      alpha-satellite DNA probes. Two gastric carcinoma cell lines, TMK-1 and MKN-28, 
      were first analyzed cytogenetically. Of 25 TMK-1 cell karyotypes, chromosome 7 
      showed trisomy and chromosome 17 showed disomy in 18 cells. Most MKN-28 cells 
      showed disomy of both chromosomes 7 and 17. Suspensions of singly isolated TMK-1 
      and MKN-7 cells were obtained from the cultured cells, and from paraffin-embedded 
      tissue specimens fixed with formalin for 0, 1, 3 and 5 days obtained from 
      xenotransplanted tumors in nude mice. The numbers of chromosomes 7 and 17 
      analyzed with the karyotypic preparations coincided well with those determined by 
      FISH, even in the paraffin-embedded specimens. The number of tumor cells showing 
      no signals, however, increased in the specimens after 5 days formalin fixation. 
      In 10 surgically removed gastric carcinomas, the predominant signal number for 
      chromosomes 7 and 17 in the cells of paraffin-embedded tissues was two (disomy), 
      except in one papillary carcinoma, which was trisomic for chromosome 7. Large 
      subpopulations (more than 20%) showing trisomy were found in four cases for 
      chromosome 7 and in five cases for chromosome 17. A higher frequency of trisomy 
      was found in well differentiated than in poorly differentiated carcinomas. These 
      findings suggest that the FISH technique is a useful tool for detecting 
      chromosomal aberrations in gastric adenocarcinoma cells, even in 
      paraffin-embedded specimens, as long as the tissues are fixed with formalin for 
      an appropriate time.
FAU - Gomyo, Y
AU  - Gomyo Y
AD  - First Department of Pathology, Faculty of Medicine, Tottori University, Yonago, 
      Japan.
FAU - Andachi, H
AU  - Andachi H
FAU - Nagao, K
AU  - Nagao K
FAU - Ikeguchi, M
AU  - Ikeguchi M
FAU - Ito, H
AU  - Ito H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Transplantation
MH  - Paraffin Embedding
MH  - Stomach Neoplasms/*genetics
MH  - Tissue Fixation
MH  - Tumor Cells, Cultured
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1995.tb03446.x [doi]
PST - ppublish
SO  - Pathol Int. 1995 Mar;45(3):227-32. doi: 10.1111/j.1440-1827.1995.tb03446.x.