PMID- 7789190
OWN - NLM
STAT- MEDLINE
DCOM- 19950726
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 70
IP  - 3-4
DP  - 1995
TI  - Molecular characterization and refined genomic localization of three human 
      potassium ion channel genes.
PG  - 280-4
AB  - Potassium ion (K+) channels are essential for a variety of cellular functions in 
      both excitable and non-excitable cells and are likely to be involved in the 
      pathogenesis of some cardiovascular and neurological disorders. To be useful in 
      candidate gene analysis of inherited diseases it is important to identify new K+ 
      channel genes and localize these sequences on the human physical and genetic 
      maps. Using fluorescence in situ hybridization (FISH), we mapped two new K+ 
      channel gene containing cosmids, c2-3a and c9-2a, to chromosomes 1 and 19, 
      respectively. Partial DNA sequencing (c2-3a) and restriction enzyme site analysis 
      (c9-2a) established the uniqueness of each clone. We refined the localization of 
      c2-3a, c9-2a and a previously described K+ channel gene KCNA5, (c7-2), by 
      performing contour length measurements of hybridized metaphase chromosomes and 
      determining the average FLpter% value (fractional length relative to the fixed 
      reference point pter x 100%). When compared to ideograms of banded metaphase 
      chromosomes, these FLpter% values correspond to 12p13.31-->p13.33, 1p13.1-->p21.1 
      and 19q13.32-->q13.33, respectively. Using FISH, each of these clones has been 
      finely mapped to a different human chromosome indicating a significant dispersion 
      of K+ channel sequences in the human genome.
FAU - Landes, G M
AU  - Landes GM
AD  - Department of Human Genetics, Integrated Genetics Inc., Framingham, MA 01701, 
      USA.
FAU - Curran, M E
AU  - Curran ME
FAU - Keating, M T
AU  - Keating MT
LA  - eng
SI  - GENBANK/S77547
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Potassium Channels)
SB  - IM
GS  - KCNA3
GS  - KCNA5
GS  - KCNC4
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 19
MH  - Cloning, Molecular
MH  - Cosmids
MH  - Genomic Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocyte Activation
MH  - Lymphocytes/cytology/immunology/metabolism
MH  - Molecular Sequence Data
MH  - Potassium Channels/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000134052 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;70(3-4):280-4. doi: 10.1159/000134052.