PMID- 7789975
OWN - NLM
STAT- MEDLINE
DCOM- 19950721
LR  - 20190904
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 25
IP  - 2
DP  - 1995 Jan 20
TI  - Region-specific cosmids and STRPs identified by chromosome microdissection and 
      FISH.
PG  - 413-20
AB  - A strategy for identifying short tandem repeat (STR)-containing cosmid clones 
      from a specific chromosomal region is described. The approach is based on the use 
      of uncloned, PCR-amplified DNA derived from chromosome microdissection and pooled 
      groups of STR sequences as hybridization probes to screen a cosmid library. 
      Cosmid clones that display a positive signal common to both hybridizations are 
      then characterized for repeat length polymorphisms. This method has been applied 
      to chromosome bands 17q12-q21, a region that includes a gene (BRCA1) involved in 
      early onset familial breast and ovarian cancer. Of 1536 chromosome 17-specific 
      cosmid clones tested, 38 were identified by the dual screening procedure. 
      Fluorescence in situ hybridization revealed that 19 cosmids originated from the 
      microdissected target region. Thirteen of the 19 cosmids were mapped between 
      markers flanking the BRCA1 region and selected for further characterization. 
      Tetranucleotide repeats were identified in 10 of these 13 cosmids. Primers 
      designed for each marker were tested on a panel of 80 CEPH parents for allele 
      sizes, frequencies, and observed heterozygosities. From these studies six 
      polymorphic and one nonpolymorphic STRs were identified. A similar approach 
      should be applicable for screening whole genomic or chromosome-specific cosmid 
      libraries in efforts to isolate new polymorphic markers from any chromosomal 
      region of interest.
FAU - Flejter, W L
AU  - Flejter WL
AD  - Department of Pediatrics, University of Michigan, Ann Arbor 48109, USA.
FAU - Bennett-Baker, P
AU  - Bennett-Baker P
FAU - Barcroft, C L
AU  - Barcroft CL
FAU - Kiousis, S
AU  - Kiousis S
FAU - Chamberlain, J S
AU  - Chamberlain JS
LA  - eng
GR  - 3 P30 HG00209/HG/NHGRI NIH HHS/United States
GR  - MO1 RR 00042/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Oligonucleotide Probes)
SB  - IM
GS  - BRCA1
MH  - Base Sequence
MH  - Breast Neoplasms/genetics
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 17
MH  - *Cosmids
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Ovarian Neoplasms/genetics
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 1995/01/20 00:00
MHDA- 1995/01/20 00:01
CRDT- 1995/01/20 00:00
PHST- 1995/01/20 00:00 [pubmed]
PHST- 1995/01/20 00:01 [medline]
PHST- 1995/01/20 00:00 [entrez]
AID - 0888-7543(95)80041-J [pii]
AID - 10.1016/0888-7543(95)80041-j [doi]
PST - ppublish
SO  - Genomics. 1995 Jan 20;25(2):413-20. doi: 10.1016/0888-7543(95)80041-j.