PMID- 7789982
OWN - NLM
STAT- MEDLINE
DCOM- 19950721
LR  - 20190904
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 25
IP  - 2
DP  - 1995 Jan 20
TI  - Treatment of cells with alkaline borate buffer extends the capability of 
      interphase FISH mapping.
PG  - 485-91
AB  - Interphase fluorescence in situ hybridization (FISH) has been shown to be a means 
      to map DNA sequences relative to each other in the 100 kb to 1-2 Mb 
      genomic-separation range. At distances below 0.1 Mb, probe sites are infrequently 
      resolved in interphase chromatin. In the 0.1- to 1-Mb range, interphase chromatin 
      can be modeled as a freely flexible chain. The mean square interphase distance 
      between two probes is proportional to the genomic separation between the probes 
      on the linear DNA molecule. Above 1-2 Mb, the relationship between interphase 
      distance and genomic separation changes abruptly and appears to level off. We 
      have used alkaline-borate treatment to expand the capability of interphase FISH 
      mapping. We show here that alkaline-borate treatment increases nuclear diameter, 
      the interphase distance between probes on homologous chromosomes, and the 
      distance between probes on the same chromosome. We also show that the mean square 
      distance between hybridization sites in borate-treated nuclei is proportional to 
      genomic separation up to 4 Mb. Thus, alkaline-borate treatment enhances the 
      capability of interphase FISH mapping by increasing the absolute distance between 
      probes and extending the range of the simple relationship between interphase 
      distance and genomic separation.
FAU - Yokota, H
AU  - Yokota H
AD  - Department of Molecular Biotechnology, University of Washington, Seattle 98195, 
      USA.
FAU - van den Engh, G
AU  - van den Engh G
FAU - Mostert, M
AU  - Mostert M
FAU - Trask, B J
AU  - Trask BJ
LA  - eng
GR  - HG00256/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Borates)
RN  - 0 (Buffers)
RN  - 0 (Oligonucleotide Probes)
RN  - 91MBZ8H3QO (sodium borate)
SB  - IM
MH  - Borates/*pharmacology
MH  - Buffers
MH  - Cells, Cultured
MH  - Cosmids
MH  - Fibroblasts/drug effects/ultrastructure
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Oligonucleotide Probes
EDAT- 1995/01/20 00:00
MHDA- 1995/01/20 00:01
CRDT- 1995/01/20 00:00
PHST- 1995/01/20 00:00 [pubmed]
PHST- 1995/01/20 00:01 [medline]
PHST- 1995/01/20 00:00 [entrez]
AID - 0888-7543(95)80049-R [pii]
AID - 10.1016/0888-7543(95)80049-r [doi]
PST - ppublish
SO  - Genomics. 1995 Jan 20;25(2):485-91. doi: 10.1016/0888-7543(95)80049-r.