PMID- 7790774
OWN - NLM
STAT- MEDLINE
DCOM- 19950721
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 57
IP  - 6
DP  - 1995 Jun
TI  - Influence of serum on zinc, toxic shock syndrome toxin-1, and 
      lipopolysaccharide-induced production of IFN-gamma and IL-1 beta by human 
      mononuclear cells.
PG  - 904-8
AB  - Measurement of cytokine secretion in vitro is usually performed in culture medium 
      supplemented with human serum. We compared the secretion of interferon-gamma and 
      interleukin-1 beta as a parameter for lymphocyte and monocyte activation in RPMI 
      1640 medium supplemented with fetal calf or autologous serum in serum-free medium 
      and protein-free medium. Four different stimulatory mechanisms were tested: 
      phytohemagglutinin, toxic shock syndrome toxin-1 (TSST-1), lipopolysaccharide 
      (LPS), and zinc ions. We found that the optimal stimulatory zinc concentration 
      depended on the total protein content of the medium, whereas the monokine levels 
      were dependent on the concentration of transport proteins such as transferrin. 
      Monokine induction by LPS or TSST-1 were each influenced by the protein and serum 
      composition in a specific manner. Our results show that the differing mechanisms 
      of cytokine induction are influenced by the medium and serum composition in a 
      diverse but specific manner. Serum- or protein-free medium are especially 
      suitable after superantigen challenge when LPS activity needs to be ruled out or 
      after activation by agents with only a weak stimulatory capacity.
FAU - Driessen, C
AU  - Driessen C
AD  - Institute of Immunology and Transfusion Medicine, University of Lubeck School of 
      Medicine, Germany.
FAU - Hirv, K
AU  - Hirv K
FAU - Wellinghausen, N
AU  - Wellinghausen N
FAU - Kirchner, H
AU  - Kirchner H
FAU - Rink, L
AU  - Rink L
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Bacterial Toxins)
RN  - 0 (Culture Media)
RN  - 0 (Enterotoxins)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 82115-62-6 (Interferon-gamma)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - *Bacterial Toxins
MH  - *Blood Physiological Phenomena
MH  - Culture Media
MH  - Enterotoxins/*pharmacology
MH  - Humans
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-1/*biosynthesis
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - *Superantigens
MH  - Zinc/*pharmacology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1002/jlb.57.6.904 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1995 Jun;57(6):904-8. doi: 10.1002/jlb.57.6.904.