PMID- 7790992
OWN - NLM
STAT- MEDLINE
DCOM- 19950727
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 175
IP  - 4
DP  - 1995 Apr
TI  - Interphase cytogenetic analysis of distinct X-chromosomal translocation 
      breakpoints in synovial sarcoma.
PG  - 391-6
AB  - Synovial sarcomas show a specific translocation involving chromosomes X and 18, 
      t(X;18)(p11.2;q11.2). Two distinct X-chromosomal breakpoints occur in different 
      synovial sarcoma tumour samples. These breakpoints are located within two related 
      genomic regions containing ornithine aminotransferase-like sequences, termed 
      OATL1 and OATL2. Preliminary observations indicated the potential correlation of 
      OATL1-associated breakpoints with biphasic tumours and OATL2-associated 
      breakpoints with monophasic fibrous tumours. The present study uses interphase 
      cytogenetics to investigate the nature of chromosomal aberrations in frozen 
      synovial sarcoma tissue samples. Two-colour fluorescence in situ hybridization 
      (FISH) was performed using probes specific for the centromeres of chromosome X or 
      18, along with yeast artificial chromosome probes corresponding to the distinct 
      breakpoint regions on Xp. One monophasic epithelial and two monophasic fibrous 
      synovial sarcomas showed an OATL2-associated breakpoint, while a biphasic tumour 
      revealed a hybridization pattern indicating a breakpoint within the OATL1 region. 
      These results confirm our previous suggestion of a relationship between 
      alternative breakpoints in Xp11.2 and different histological phenotypes observed 
      in synovial sarcomas. They also demonstrate the utility of the two-colour 
      hybridization approach for the identification of chromosomal changes in 
      interphase nuclei isolated from frozen tissues.
FAU - Janz, M
AU  - Janz M
AD  - Institute for Molecular Biology, Hannover Medical School, Germany.
FAU - De Leeuw, B
AU  - De Leeuw B
FAU - Weghuis, D O
AU  - Weghuis DO
FAU - Werner, M
AU  - Werner M
FAU - Nolte, M
AU  - Nolte M
FAU - Geurts Van Kessel, A
AU  - Geurts Van Kessel A
FAU - Nordheim, A
AU  - Nordheim A
FAU - Hipskind, R A
AU  - Hipskind RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - *Chromosomes, Human, Pair 18
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Sarcoma, Synovial/*genetics/metabolism
MH  - *Translocation, Genetic
MH  - *X Chromosome
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1002/path.1711750405 [doi]
PST - ppublish
SO  - J Pathol. 1995 Apr;175(4):391-6. doi: 10.1002/path.1711750405.