PMID- 7792139
OWN - NLM
STAT- MEDLINE
DCOM- 19950727
LR  - 20191210
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 429
IP  - 5
DP  - 1995 Mar
TI  - Control of pulmonary vascular smooth muscle tone by sarcoplasmic reticulum Ca2+ 
      pump blockers: thapsigargin and cyclopiazonic acid.
PG  - 617-24
AB  - The effect of thapsigargin (TG) and cyclopiazonic acid (CPA) on the mechanical 
      activity of the rat pulmonary artery were investigated. In chemically 
      (beta-escin)-skinned arterial strips, application of TG (0.1-1 microM) or CPA 
      (0.5-10 microM) prior and throughout the loading procedure of the internal Ca2+ 
      stores (0.3 microM free Ca2+ ions for 8-10 min) concentration dependently 
      inhibited the subsequent contractile response induced by noradrenaline (NA, 10 
      microM) or caffeine (25 mM). In intact strips repeatedly incubated in a 
      Ca(2+)-containing solution (2.5 mM for 10 min), followed by incubation in a 
      Ca(2+)-free solution 12 min before NA-stimulation, TG and CPA not only inhibited 
      the NA-induced contraction but also increased the tension which appeared during 
      the exposure time to Ca2+. The two phenomena developed with similar time courses. 
      The increase in tension during the readmission of Ca2+ ions was not antagonized 
      by verapamil (10 microM) or nifedipine (1 microM) but was blocked by La3+ (50 
      microM) and Co2+ (1 mM) ions. The amplitude of the verapamil-insensitive TG (or 
      CPA)-induced contraction was dependent on the external [Ca2+] [0.1-10 mM, 
      concentration for half maximal effect (EC50) = 0.85 mM], not modified by the 
      reduction of the external [Na+] (from 130 to 10 mM) and decreased by 
      depolarization of the strip using K(+)-rich (30-120 mM) solutions. Under the 
      latter condition, 38 +/- 9 and 83 +/- 4% reduction (n = 5) was observed in the 
      presence of 60 and 120 mM K+ respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Gonzalez De La Fuente, P
AU  - Gonzalez De La Fuente P
AD  - Laboratoire de Physiologie, Universite de Bordeaux 2, France.
FAU - Savineau, J P
AU  - Savineau JP
FAU - Marthan, R
AU  - Marthan R
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Indoles)
RN  - 0 (Terpenes)
RN  - 3G6A5W338E (Caffeine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6805-41-0 (Escin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - X9TLY4580Z (cyclopiazonic acid)
RN  - YZS2RPE8LE (Strontium)
SB  - IM
MH  - Animals
MH  - Caffeine/pharmacology
MH  - Calcium/metabolism
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Escin/pharmacology
MH  - In Vitro Techniques
MH  - Indoles/*pharmacology
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle Tonus/*drug effects
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - Norepinephrine/pharmacology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors
MH  - Pulmonary Artery/cytology/drug effects
MH  - Pulmonary Circulation/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Sarcoplasmic Reticulum/drug effects/*metabolism
MH  - Strontium/pharmacology
MH  - Terpenes/*pharmacology
MH  - Thapsigargin
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1007/BF00373982 [doi]
PST - ppublish
SO  - Pflugers Arch. 1995 Mar;429(5):617-24. doi: 10.1007/BF00373982.