PMID- 7794115
OWN - NLM
STAT- MEDLINE
DCOM- 19950727
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 140
IP  - 4
DP  - 1995
TI  - Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine 
      reproductive and respiratory syndrome virus (PRRSV): implication for the 
      existence of two genotypes of PRRSV in the U.S.A. and Europe.
PG  - 745-55
AB  - The putative membrane (M) protein (ORF 6) and nucleocapsid (N) protein (ORF 7) 
      genes of five U.S. isolates of porcine reproductive and respiratory syndrome 
      virus (PRRSV) with differing virulence were cloned and sequenced. To determine 
      the genetic variation and the phylogenetic relationship of PRRSV, the deduced 
      amino acid sequences of the putative M and N proteins from these isolates were 
      aligned, to the extent known, with other PRRSV isolates, and also other members 
      of the proposed arterivirus group including lactate dehydrogenase-elevating virus 
      (LDV) and equine arteritis virus (EAV). There was 96-100% amino acid sequence 
      identity in the putative M and N genes among U.S. and Canadian PRRSV isolates 
      with differing virulence. However, their amino acid sequences varied extensively 
      from those of European PRRSV isolates, and displayed only 57-59% and 78-81% 
      identity, respectively. The phylogenetic trees constructed on the basis of the 
      putative M and N genes of the proposed arterivirus group were similar and 
      indicated that both U.S. and European PRRSV isolates were related to LDV and were 
      distantly related to EAV. The U.S. and European PRRSV isolates fell into two 
      distinct groups, suggesting that U.S. and European PRRSV isolates represent two 
      distinct genotypes.
FAU - Meng, X J
AU  - Meng XJ
AD  - Veterinary Medical Research Institute, Iowa State University, Ames, USA.
FAU - Paul, P S
AU  - Paul PS
FAU - Halbur, P G
AU  - Halbur PG
FAU - Lum, M A
AU  - Lum MA
LA  - eng
SI  - GENBANK/U18748
SI  - GENBANK/U18749
SI  - GENBANK/U18750
SI  - GENBANK/U18751
SI  - GENBANK/U18752
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arterivirus/*classification/genetics
MH  - Base Sequence
MH  - Capsid/*genetics
MH  - DNA Primers
MH  - DNA, Viral
MH  - Europe
MH  - Genes, Viral
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Swine
MH  - United States
MH  - Viral Core Proteins/*genetics
MH  - Viral Matrix Proteins/*genetics
PMC - PMC7086766
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 2020/03/23
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - BF01309962 [pii]
AID - 10.1007/BF01309962 [doi]
PST - ppublish
SO  - Arch Virol. 1995;140(4):745-55. doi: 10.1007/BF01309962.