PMID- 7795734
OWN - NLM
STAT- MEDLINE
DCOM- 19950803
LR  - 20061115
IS  - 0914-8108 (Print)
IS  - 0914-8108 (Linking)
VI  - 12
IP  - 1
DP  - 1995 Mar
TI  - Cytogenical analysis of brain tumors by FISH (fluorescence in situ hybridization) 
      and FCM (flow cytometry).
PG  - 75-82
AB  - In the present study, an analysis was made of chromosomal aberrations in brain 
      tumors using the fluorescence in situ hybridization (FISH) technique. At the same 
      time DNA histograms were obtained by flow cytometry (FCM) to make a comparative 
      study of histological malignancy and prognosis. The subjects included 30 gliomas 
      (7 of astrocytoma grade II, 15 of anaplastic astrocytoma (grade III), 8 of 
      glioblastoma (grade IV)) and 26 meningiomas. In the study of FISH, DNA probes for 
      chromosomes No. 7, 9, 10, and 17, were used to cause a reaction with chromosome 
      No. 22 added for meningiomas. In the study with FCM, DNA index from DNA histogram 
      was calculated using lymphocytes as the internal standard. In gliomas as a whole, 
      chromosomes No. 7 and 17 showed high values, whereas, chromosomes No. 9 and 10 
      low values. Analysis by the grades of glioma showed that compared with gliomas of 
      other grades, grade IV gliomas were higher for chromosome No. 17 and lower for 
      chromosome No. 10. In meningiomas, while many cases showed a low value for 
      chromosome No. 22, most cases of recurrent and atypical meningiomas showed a high 
      value for chromosome No. 17. In gliomas, DNA index showed a correlation with the 
      grade, and a positive correlation particularly with chromosome No. 17 in FISH. 
      Recurrent and atypical meningiomas had a high DNA index.
FAU - Kasai, H
AU  - Kasai H
AD  - Department of Neurosurgery, Kansai Medical University.
FAU - Kawamoto, K
AU  - Kawamoto K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Noshuyo Byori
JT  - Noshuyo byori = Brain tumor pathology
JID - 9315330
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Neoplasms/*genetics/pathology
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Flow Cytometry
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meningioma/*genetics/immunology
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Ploidies
MH  - Prognosis
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
PST - ppublish
SO  - Noshuyo Byori. 1995 Mar;12(1):75-82.