PMID- 7796605
OWN - NLM
STAT- MEDLINE
DCOM- 19950801
LR  - 20190830
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 14
IP  - 3
DP  - 1995 Mar
TI  - A new fluorescent imaging procedure in vivo for evaluation of the retinal 
      microcirculation in rats.
PG  - 223-8
AB  - We investigated a new method for in vivo evaluation of the retinal 
      microcirculation in rats using a cell-permeant fluorescent dye, acridine orange 
      (AO), which stains cell nuclei and cytoplasm, and a scanning laser ophthalmoscope 
      (SLO). AO, which binds and interacts with DNA and RNA, and thus stains cell 
      nuclei and cytoplasm, was administered intravenously to rats. Fluorescein 
      angiography was performed after administration of the AO, and fundus images were 
      recorded on S-VHS videotape by means of an SLO. Argon laser was used as an 
      exciter of the dye. The retinal vessels were stained with the dye, rendering the 
      retinal microvasculature clearly visible. Cell nuclei and vessel walls were 
      observed as greater fluorescence and lesser fluorescence, respectively. 
      Leukocytes were also observed as highly fluorescent dots moving through the 
      vessels. The results suggest that SLO visualization of AO uptake by cells may be 
      a useful procedure for the evaluation of retinal microcirculation in vivo in 
      rats.
FAU - Kimura, H
AU  - Kimura H
AD  - Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
FAU - Kiryu, J
AU  - Kiryu J
FAU - Nishiwaki, H
AU  - Nishiwaki H
FAU - Ogura, Y
AU  - Ogura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange/administration & dosage
MH  - Animals
MH  - Blood Flow Velocity
MH  - Cell Nucleus
MH  - Endothelium, Vascular/cytology
MH  - Fluorescein Angiography/*methods
MH  - Fundus Oculi
MH  - Injections, Intravenous
MH  - Lasers
MH  - Leukocytes/cytology
MH  - Male
MH  - Microcirculation
MH  - Ophthalmoscopes
MH  - Rats
MH  - Retinal Vessels/*physiology
MH  - Videotape Recording
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.3109/02713689509033518 [doi]
PST - ppublish
SO  - Curr Eye Res. 1995 Mar;14(3):223-8. doi: 10.3109/02713689509033518.