PMID- 7797472
OWN - NLM
STAT- MEDLINE
DCOM- 19950731
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 25
DP  - 1995 Jun 23
TI  - Platelet-activating factor induces NF-kappa B activation through a G 
      protein-coupled pathway.
PG  - 14928-34
AB  - The capability of platelet-activating factor (PAF) to induce transcription factor 
      activation was examined. In stably transfected Chinese hamster ovary cells 
      expressing the PAF receptor (CHO-PAFR), PAF stimulation resulted in the nuclear 
      expression of a DNA binding activity with specificity to the kappa B sequence. 
      The p50 and p65 proteins, constituents of the prototypic nuclear factor kappa B 
      (NF-kappa B), were identified as components of the DNA protein complexes by 
      antipeptide antibodies in gel supershift as well as UV cross-linking experiments. 
      PAF induced an initial decrease and subsequent increase of cytoplasmic I kappa B 
      alpha levels, accompanied by up-regulation of the I kappa B alpha messenger RNA, 
      a feature of NF-kappa B activation. PAF-induced kappa B binding activity was 
      detected within 15 min after agonist stimulation, peaked at 30-40 min, and 
      remained detectable by 2.5 h. SR 27417, a PAF receptor antagonist, blocked 
      PAF-induced kappa B binding activity but not that induced by tumor necrosis 
      factor-alpha (TNF alpha). Cholera toxin treatment markedly reduced PAF-induced 
      kappa B binding activity, whereas pertussis toxin had no significant inhibitory 
      effect. Neither of the two toxins affected the kappa B binding activity induced 
      by TNF alpha in the same cells. In addition to the CHO-PAFR cells, PAF stimulated 
      kappa B binding activity in the murine P388D1 macrophage and the human ASK.0 B 
      cell lines that express endogenous PAF receptors. These results imply a potential 
      role of PAF in the regulation of gene expression through a G protein-coupled 
      transcription factor activation pathway.
FAU - Kravchenko, V V
AU  - Kravchenko VV
AD  - Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.
FAU - Pan, Z
AU  - Pan Z
FAU - Han, J
AU  - Han J
FAU - Herbert, J M
AU  - Herbert JM
FAU - Ulevitch, R J
AU  - Ulevitch RJ
FAU - Ye, R D
AU  - Ye RD
LA  - eng
GR  - AI15136/AI/NIAID NIH HHS/United States
GR  - AI33503/AI/NIAID NIH HHS/United States
GR  - GM37696/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (NF-kappa B)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (platelet activating factor receptor)
RN  - 9007-49-2 (DNA)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - CHO Cells
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cricetinae
MH  - DNA/chemistry/metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Kinetics
MH  - Macrophages
MH  - Mice
MH  - Molecular Sequence Data
MH  - NF-kappa B/*biosynthesis/metabolism
MH  - Oligodeoxyribonucleotides
MH  - Platelet Activating Factor/metabolism/*pharmacology
MH  - Platelet Membrane Glycoproteins/biosynthesis/*physiology
MH  - Promoter Regions, Genetic
MH  - *Receptors, Cell Surface
MH  - *Receptors, G-Protein-Coupled
MH  - Recombinant Proteins/biosynthesis/metabolism
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1995/06/23 00:00
MHDA- 1995/06/23 00:01
CRDT- 1995/06/23 00:00
PHST- 1995/06/23 00:00 [pubmed]
PHST- 1995/06/23 00:01 [medline]
PHST- 1995/06/23 00:00 [entrez]
AID - S0021-9258(18)90485-0 [pii]
AID - 10.1074/jbc.270.25.14928 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Jun 23;270(25):14928-34. doi: 10.1074/jbc.270.25.14928.