PMID- 7798796
OWN - NLM
STAT- MEDLINE
DCOM- 19950124
LR  - 20131121
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 124
IP  - 6
DP  - 1994 Dec
TI  - Species diversity of 11 beta-hydroxysteroid dehydrogenase in the cardiovascular 
      system.
PG  - 821-6
AB  - Mineralocorticoid (MC) receptors are found in classic (e.g., kidney) and 
      nonclassic (e.g., heart and aorta) tissues. MC receptor specificity at either 
      site is conferred by the enzyme 11 beta-hydroxysteroid dehydrogenase (11 
      beta-HSD), which metabolizes glucocorticoids to inactive 11-ketosteroids. Because 
      the heart and vasculature may be target tissues for aldosterone, this study was 
      undertaken. Its objectives were to measure 11 beta-HSD at these sites and to 
      compare levels of activity in the atria and ventricles of different species. 
      Toward this end we first determined levels of plasma corticosterone (B) and 
      cortisol (F) (by using radioimmunoassay), in a variety of species, for subsequent 
      correlation with cardiac 11 beta-HSD activity. 11 beta-Dehydrogenation of 
      glucocorticoids was then assayed in ventricles and atria as well as aorta. Tissue 
      homogenates containing 1 to 5 mg protein were incubated for 1 hour in the 
      presence of 1 muCi 5 x 10(-9) mol/L tritiated B or tritiated F and 5 x 10(-4) 
      mol/L oxidized nicotinamide adenine dinucleotide phosphate. Steroid separation 
      and quantitation were achieved by using reverse-phase high-performance liquid 
      chromatography coupled to an online radioisotope detector. For species in which B 
      circulates at relatively high concentrations (rat, rabbit, pig), high levels of 
      dehydrogenation of B to 11-dehydrocorticosterone (A) were observed in both atria 
      and ventricles. Overall, cardiac B to A conversion levels corresponded to between 
      0.3 and 0.4 pmol A formed/mg protein/hr. 11 beta-HSD activity was also detected 
      in the aorta.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Slight, S
AU  - Slight S
AD  - Department of Internal Medicine, University of Missouri-Columbia 65212.
FAU - Ganjam, V K
AU  - Ganjam VK
FAU - Weber, K T
AU  - Weber KT
LA  - eng
GR  - R01-HL-31701/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Glucocorticoids)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - V27W9254FZ (Cortisone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Aorta/metabolism
MH  - Cardiovascular System/*enzymology
MH  - Cattle
MH  - Cortisone/blood
MH  - Dogs
MH  - Glucocorticoids/metabolism
MH  - Heart Atria
MH  - Heart Ventricles
MH  - Horses
MH  - Humans
MH  - Hydrocortisone/blood
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Myocardium/metabolism
MH  - Rabbits
MH  - Rats
MH  - Species Specificity
MH  - Swine
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 0022-2143(94)90045-0 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1994 Dec;124(6):821-6.