PMID- 7798893
OWN - NLM
STAT- MEDLINE
DCOM- 19950120
LR  - 20190909
IS  - 0162-0134 (Print)
IS  - 0162-0134 (Linking)
VI  - 56
IP  - 2
DP  - 1994 Nov 1
TI  - Enhanced synthesis of metallothionein as a possible cause of abnormal copper 
      accumulation in LEC rats.
PG  - 117-25
AB  - Long-Evans rats with a cinnamon-like coat color (LEC) is an inbred strain 
      accumulating copper (Cu) in the liver abnormally and showing spontaneous 
      hepatitis and hepatoma. The present study was intended to clarify how Cu 
      accumulates in the LEC rat liver. For this purpose, the distribution profiles of 
      Cu and zinc (Zn) and the inducibility of metallothionein (MT) synthesis were 
      examined in the liver between Cu-loaded Long Evans agouti (LEA, the original 
      strain of LEC) rats and were compared with those in control LEC rats. LEA rats 
      (female, five weeks old) were injected subcutaneously with CuCl2 daily at a dose 
      of 3 mg Cu/kg body weight for 2, 4, 6, and 9 days. The concentration of Cu (124 
      micrograms/g) accumulated in the LEA rat liver after four injections was 
      comparable to that in control LEC rats. Only 20% of Cu in the liver of LEA rats 
      was recovered in the supernatant fraction in the form of MT, while Cu in the LEC 
      rat liver (113 micrograms/g) was recovered mostly in the supernatant fraction, 
      and was bound to MT. Although the increased concentration of Cu in the LEA rat 
      liver was further elevated after additional injections of Cu, the amount of MT 
      did not increase further. The MT mRNA content in the LEA rat liver remained lower 
      than that of LEC rats even after further injections of Cu. Therefore, the present 
      results suggest that LEC rats can accumulate Cu at a high concentration in the 
      liver because of their extremely high inducibility of MT.
FAU - Kanno, S
AU  - Kanno S
AD  - National Institute for Environmental Studies, Tsukuba Japan.
FAU - Aoki, Y
AU  - Aoki Y
FAU - Suzuki, J S
AU  - Suzuki JS
FAU - Takeichi, N
AU  - Takeichi N
FAU - Misawa, S
AU  - Misawa S
FAU - Suzuki, K T
AU  - Suzuki KT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (RNA, Messenger)
RN  - 789U1901C5 (Copper)
RN  - 9038-94-2 (Metallothionein)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Copper/analysis/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Hair Color
MH  - Liver/chemistry/*metabolism
MH  - Metallothionein/*biosynthesis
MH  - Protein Binding
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Species Specificity
MH  - Zinc/analysis/metabolism
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 0162-0134(94)85042-9 [pii]
AID - 10.1016/0162-0134(94)85042-9 [doi]
PST - ppublish
SO  - J Inorg Biochem. 1994 Nov 1;56(2):117-25. doi: 10.1016/0162-0134(94)85042-9.