PMID- 7799168
OWN - NLM
STAT- MEDLINE
DCOM- 19950125
LR  - 20031114
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 80
IP  - 6
DP  - 1994 Dec
TI  - Localization of phenol oxidase in female Trichuris suis.
PG  - 952-9
AB  - A phenol oxidase (E.C. 1.10.3.1) preparation from adult female Trichuris suis was 
      assayed by both polarographic and spectrophotometric techniques. The T. suis 
      enzyme oxidized most diphenols including 4-methylcatechol (4MC) and 
      dihydroxyphenylalanine but did not oxidize tyrosine. The pH and temperature 
      optima were 6.8 and 36 C, respectively. The Km measured using 4MC as a substrate 
      ranged from 0.12 to 0.4 mM. The highest phenol oxidase activity was isolated in 
      fractions from the adult females that were enriched in eggs relative to the 
      activity in somatic tissue from females and all male tissues that were assayed. 
      Phenol oxidase activity was localized on sodium dodecyl sulfate-polyacrylamide 
      gel electrophoresis substrate gels into 2 bands with M(r)'s of 44,000 and 53,000. 
      An antibody to the 44,000 band recognized 2 bands of 40,000 and 45,000 M(r) on 
      western blot analysis of the enzyme preparation. Immunocytochemical localization 
      of anti-phenol oxidase antibody in serial cross sections of adult female worms 
      indicates that the enzyme is found exclusively in the anterior part of the 
      parasite in the proximal part of the uterus that is posterior to the junction 
      with the stichosome. Eggs located in more distal parts of the reproductive system 
      did not react with the antibody. The results indicate that a phenol oxidase is 
      located in the fertilized eggs of adult female T. suis. It is likely that phenol 
      oxidase contributes significantly to the chemical hardening process in the eggs 
      when they pass out into the external environment. Inhibition of phenol oxidase 
      may reduce the survivability of the eggs and thus minimize contamination of 
      livestock facilities.
FAU - Fetterer, R H
AU  - Fetterer RH
AD  - Helminthic Diseases Laboratory, U.S. Department of Agriculture, Beltsville, 
      Maryland 20705.
FAU - Hill, D E
AU  - Hill DE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Hydrogen-Ion Concentration
MH  - Immunohistochemistry
MH  - Male
MH  - Monophenol Monooxygenase/*analysis/chemistry
MH  - Oxygen Consumption
MH  - Spectrophotometry
MH  - Substrate Specificity
MH  - Swine
MH  - Temperature
MH  - Trichuris/*enzymology
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
PST - ppublish
SO  - J Parasitol. 1994 Dec;80(6):952-9.