PMID- 7802259
OWN - NLM
STAT- MEDLINE
DCOM- 19950123
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 66
IP  - 21
DP  - 1994 Nov 1
TI  - Predictive steady-state chromatography. 1. Algorithms for leading and trailing 
      edges of resolved and unresolved peaks in liquid chromatography.
PG  - 3765-72
AB  - This paper describes mathematical models and curve-fitting procedures that permit 
      steady-state saturation signals to be computed accurately from data along leading 
      and trailing edges of liquid chromatograms. This new approach to quantitative 
      chromatography is called predictive steady-state chromatography (PSSC). It is 
      shown that the computed saturation signals are virtually the same when determined 
      from data along leading and trailing edges and they vary linearly with analyte 
      concentration. Most importantly, the computed saturation signals for a given 
      analyte concentration are virtually independent of experimental variables such as 
      sample volume and flow rate. For example, for sample volumes between 25 and 45 
      microL, the average computed saturation signal for a 0.025 mM solution of 
      theophylline was 0.11 V with a standard deviation of 0.00097 V (RSD = 0.8%); 
      similar results were found for other concentrations and for changes in flow rate. 
      Dependencies of the PSSC method on sample volume and flow rate were compared with 
      dependencies for peak-height and peak-area methods by using relative error 
      coefficients. Dependencies on sample volume were 0.04%/microL for the PSSC method 
      and 3 and 4%/microL for peak-height and peak-area methods, respectively. 
      Dependencies on flow rate were 2%/mL/min for the PSSC method and 17 and 
      120%/mL/min for the peak-height and peak-area methods, respectively. Thus, the 
      predictive steady-state method is 10-100-fold more rugged than peak-height and 
      peak-area methods.
FAU - Li, J
AU  - Li J
AD  - Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393.
FAU - Pardue, H L
AU  - Pardue HL
LA  - eng
GR  - GM 13326-25/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 3G6A5W338E (Caffeine)
RN  - C137DTR5RG (Theophylline)
SB  - IM
MH  - Algorithms
MH  - Caffeine/analysis/*chemistry
MH  - Chromatography, Liquid/*standards
MH  - Computer Simulation
MH  - Models, Theoretical
MH  - Reproducibility of Results
MH  - Theophylline/analysis/*chemistry
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1021/ac00093a036 [doi]
PST - ppublish
SO  - Anal Chem. 1994 Nov 1;66(21):3765-72. doi: 10.1021/ac00093a036.