PMID- 7803279
OWN - NLM
STAT- MEDLINE
DCOM- 19950202
LR  - 20211203
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 88
IP  - 2
DP  - 1994 Oct
TI  - Sequential acquisition of trisomy 8 and N-ras mutation in acute myeloid leukaemia 
      demonstrated by analysis of isolated leukaemic colonies.
PG  - 338-42
AB  - Specific chromosomal aberrations and point mutations of the N-ras proto-oncogene 
      are characteristic genetic alterations in acute leukaemias. However, the 
      relationships between these two different genetic changes are unclear. Here we 
      have determined the order of genetic events in a patient with acute myeloid 
      leukaemia characterized by trisomy 8 and a point mutation of N-ras at codon 12 
      (N12-cys) and codon 61 (N61-his). 30 colonies obtained by in vitro clonogenic 
      assay of leukaemic cells from a patient with AML were individually analysed for 
      the presence of trisomy 8 and each of two different N-ras mutations by 
      fluorescence in situ hybridization (FISH) and the polymerase chain reaction 
      (PCR). Trisomy 8 was detected in 25/26 evaluable colonies. 19/26 colonies 
      contained the N12-cys mutation. The N61-his mutation was not detected in any of 
      the colonies obtained. All the colonies with the N12 cys mutation were also 
      trisomic from chromosome 8, whereas 6/25 colonies with trisomy 8 had no N-ras 
      mutation. These data suggest that trisomy 8 was acquired before N12 cys mutation 
      in the pathogenesis of this leukaemia and that two genetic events can co-operate 
      within a single subclone.
FAU - Price, C M
AU  - Price CM
AD  - Centre of Cell and Molecular Biology, Chester Beatty Laboratories, Institute of 
      Cancer Research, London.
FAU - Marshall, C J
AU  - Marshall CJ
FAU - Bashey, A
AU  - Bashey A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Genes, ras/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelomonocytic, Acute/*genetics
MH  - Molecular Sequence Data
MH  - Neoplastic Stem Cells/physiology
MH  - *Point Mutation
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Mas
MH  - Time Factors
MH  - *Trisomy
MH  - Tumor Cells, Cultured
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1994.tb05028.x [doi]
PST - ppublish
SO  - Br J Haematol. 1994 Oct;88(2):338-42. doi: 10.1111/j.1365-2141.1994.tb05028.x.