PMID- 7803405
OWN - NLM
STAT- MEDLINE
DCOM- 19950127
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 33
IP  - 51
DP  - 1994 Dec 27
TI  - Backbone dynamics of a two-domain protein: 15N relaxation studies of the 
      amino-terminal fragment of urokinase-type plasminogen activator.
PG  - 15418-24
AB  - The amino-terminal fragment (ATF) of urokinase-type plasminogen activator (u-PA) 
      is a two-domain protein which consists of a kringle and a growth factor domain 
      (GFD). The dynamics of uniformly 15N-labeled ATF was examined by measuring the 
      longitudinal (T1) and transverse (T2) 15N relaxation times and heteronuclear 
      NOEs. The data were interpreted in terms of the model-independent spectral 
      density function. The GFD was found to exhibit a high degree of anisotropy, 
      whereas the kringle domain of ATF undergoes isotropic reorientation. This 
      difference in anisotropy is best explained by the two domains moving 
      independently such as differently shaped beads on a string. With the exception of 
      the N- and C-terminal regions of the protein, the most flexible region of ATF was 
      the seven-residue omega loop (N22-I28) of the GFD which has been implicated in 
      the binding of u-PA to its receptor. The amides of the linker region between the 
      domains displayed high values of the order parameter, indicating restricted 
      motion on the picosecond time scale. This is in contrast to the flexible linker 
      of calmodulin [Barbato et al. (1992) Biochemistry 31, 5269-5278], which displayed 
      low values of S2 and unrestricted motion in the linker region.
FAU - Hansen, A P
AU  - Hansen AP
AD  - Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Ilinois 
      60064.
FAU - Petros, A M
AU  - Petros AM
FAU - Meadows, R P
AU  - Meadows RP
FAU - Fesik, S W
AU  - Fesik SW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Kringles
MH  - Magnetic Resonance Spectroscopy
MH  - Motion
MH  - Protein Structure, Tertiary
MH  - Recombinant Proteins
MH  - Urokinase-Type Plasminogen Activator/*chemistry
EDAT- 1994/12/27 00:00
MHDA- 1994/12/27 00:01
CRDT- 1994/12/27 00:00
PHST- 1994/12/27 00:00 [pubmed]
PHST- 1994/12/27 00:01 [medline]
PHST- 1994/12/27 00:00 [entrez]
AID - 10.1021/bi00255a023 [doi]
PST - ppublish
SO  - Biochemistry. 1994 Dec 27;33(51):15418-24. doi: 10.1021/bi00255a023.