PMID- 7803609
OWN - NLM
STAT- MEDLINE
DCOM- 19950130
LR  - 20220317
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 51
IP  - 3
DP  - 1994 Sep
TI  - Chromosome mosaicism in human embryos.
PG  - 373-9
AB  - In the human, mosaicism may occur before implantation; but, to determine when it 
      first occurs, it is necessary to study the chromosomal complement of all 
      blastomeres. Full karyotypes of blastomeres from 2- to 8-cell human embryos by 
      conventional karyotyping of metaphase spreads are difficult to obtain. The aim of 
      this study was to assess the stage at which mosaicism occurred in preimplantation 
      human embryos through use of fluorescence in situ hybridization (FISH) with 
      multiple probes. All or most blastomeres from 2- to 12-cell human embryos were 
      analyzed by FISH using probes for gonosomes and chromosome 18. FISH was performed 
      on blastomeres from 117 morphologically normal monospermic embryos that were not 
      transferred after preimplantation diagnosis because of their risk of carrying 
      X-linked disease; 20 (17.1%) of these embryos were mosaic. Another group of 163 
      arrested or morphologically abnormal monospermic embryos were also analyzed by 
      FISH; 47 (28.8%) of these embryos were mosaic. In addition, 37 dispermic embryos 
      were analyzed, and 28 (75.7%) of these were found to be mosaic. Mosaicism first 
      occurred at the second cleavage division when the monospermic embryo was mostly 
      diploid and at the first cleavage division when the embryo was mostly haploid, 
      polyploid, or dispermic.
FAU - Munne, S
AU  - Munne S
AD  - Center for Reproductive Medicine and Infertility, New York Hospital-Cornell 
      University Medical Center, New York 10021.
FAU - Weier, H U
AU  - Weier HU
FAU - Grifo, J
AU  - Grifo J
FAU - Cohen, J
AU  - Cohen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
SB  - IM
MH  - Blastomeres/*ultrastructure
MH  - Chromosomes, Human, Pair 18
MH  - Female
MH  - Genetic Diseases, Inborn/genetics
MH  - Genetic Linkage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - *Mosaicism
MH  - Ploidies
MH  - Pregnancy
MH  - Prenatal Diagnosis
MH  - X Chromosome
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1095/biolreprod51.3.373 [doi]
PST - ppublish
SO  - Biol Reprod. 1994 Sep;51(3):373-9. doi: 10.1095/biolreprod51.3.373.