PMID- 7804125
OWN - NLM
STAT- MEDLINE
DCOM- 19950130
LR  - 20091119
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 12
IP  - 5
DP  - 1994 Sep
TI  - Transforming growth factor-beta 1 augments macrophage-colony stimulating factor 
      activity on human marrow.
PG  - 527-32
AB  - Transforming growth factor-beta 1 (TGF-beta 1) suppresses the colony stimulating 
      activity of most cytokines. The effect of TGF-beta 1 on macrophage colonies 
      induced by macrophage colony stimulating factor (M-CSF) from human marrow has not 
      been described. Experiments were performed with phenylalanine methyl ester (PME) 
      treated marrow. PME (5 mM) eliminates stromal cells and monocytes. Colony 
      stimulatory factors were used at plateau concentrations. TGF-beta 1 (0.1 ng/ml) 
      significantly (p < 0.05) augmented M-CSF induced macrophage colony forming units 
      (CFU-M) by twofold to fourfold in 8/8 donors. In contrast, colonies stimulated by 
      granulocyte-macrophage CSF (GM-CSF) (CFU-GM), were significantly decreased by 
      TGF-beta 1. To determine if TGF-beta 1 was present in effective concentrations in 
      vitro, cultures were performed with anti-TGF-beta 1. Anti-TGF-beta 1 decreased (p 
      < 0.05) M-CSF induced colonies in 5/6 donors. The method of TGF-beta 1 
      enhancement was explored with antihuman CSF-1 receptor antibody. Antihuman CSF-1 
      receptor antibody resulted in comparable suppression of CFU-M resulting from both 
      M-CSF and M-CSF + TGF-beta 1. These studies indicate that TGF-beta 1 directly 
      enhances M-CSF activity by a mechanism other than upregulation of M-CSF 
      receptors.
FAU - Rosenfeld, C S
AU  - Rosenfeld CS
AD  - Western Pennsylvania Cancer Institute, West Penn Hospital, Pittsburgh.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Transforming Growth Factor beta)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Bone Marrow/*drug effects
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - Drug Synergism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoietic Stem Cells/cytology/*drug effects
MH  - Humans
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Receptor, Macrophage Colony-Stimulating Factor/antagonists & 
      inhibitors/immunology
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Up-Regulation/drug effects
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1002/stem.5530120509 [doi]
PST - ppublish
SO  - Stem Cells. 1994 Sep;12(5):527-32. doi: 10.1002/stem.5530120509.