PMID- 7806232
OWN - NLM
STAT- MEDLINE
DCOM- 19950130
LR  - 20220316
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 22
IP  - 2
DP  - 1994 Jul 15
TI  - Regional localization of 725 human chromosome 7-specific yeast artificial 
      chromosome clones.
PG  - 439-48
AB  - Toward the construction of a complete physical map of human chromosome 7, we have 
      localized 725 YAC clones to cytogenetically defined regions using fluorescence in 
      situ hybridization (FISH) and by screening with DNA markers of known chromosomal 
      locations. These chromosome 7-specific YAC clones are part of a library 
      constructed with DNA isolated from monochromosomal 7 human-hamster somatic cell 
      hybrid lines. The FISH mapping for 575 clones was accomplished by using "Alu-PCR" 
      amplified YAC DNA against metaphase chromosome spreads made from a 
      monochromosomal 7 human-mouse somatic cell hybrid line. Hybridization- or 
      PCR-based screening of previously mapped DNA markers was performed for the 
      mapping of 221 YAC clones. There was excellent correlation between the map 
      locations obtained for the 71 YACs localized with both methods. All of the 
      regionally localized YAC clones are valuable reagents for mapping and 
      identification of disease genes on human chromosome 7.
FAU - Kunz, J
AU  - Kunz J
AD  - Institut fur Humangenetik, Philipps-Universitat Marburg, Germany.
FAU - Scherer, S W
AU  - Scherer SW
FAU - Klawitz, I
AU  - Klawitz I
FAU - Soder, S
AU  - Soder S
FAU - Du, Y Z
AU  - Du YZ
FAU - Speich, N
AU  - Speich N
FAU - Kalff-Suske, M
AU  - Kalff-Suske M
FAU - Heng, H H
AU  - Heng HH
FAU - Tsui, L C
AU  - Tsui LC
FAU - Grzeschik, K H
AU  - Grzeschik KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 7
MH  - Cricetinae
MH  - Gene Library
MH  - *Genetic Markers
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
EDAT- 1994/07/15 00:00
MHDA- 1994/07/15 00:01
CRDT- 1994/07/15 00:00
PHST- 1994/07/15 00:00 [pubmed]
PHST- 1994/07/15 00:01 [medline]
PHST- 1994/07/15 00:00 [entrez]
AID - S0888-7543(84)71407-8 [pii]
AID - 10.1006/geno.1994.1407 [doi]
PST - ppublish
SO  - Genomics. 1994 Jul 15;22(2):439-48. doi: 10.1006/geno.1994.1407.