PMID- 7807177
OWN - NLM
STAT- MEDLINE
DCOM- 19950130
LR  - 20190909
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 19
IP  - 3
DP  - 1994
TI  - Negative effects of wild-type p53 and s-Myc on cellular growth and tumorigenicity 
      of glioma cells. Implication of the tumor suppressor genes for gene therapy.
PG  - 259-68
AB  - Human (U251, U87, U343) and rat glioma cell lines (C6, 9L) were examined by the 
      reverse transcriptase-polymerase chain reaction and subsequent nucleotide 
      sequencing analysis to see whether they express wild type (wt)-p53 or mutated 
      form (mut)-p53 messages. Results showed that U87, U343, and C6 cells expressed 
      wt-p53 messages whereas U251 and 9L cells expressed mut-p53 messages. All these 
      cell lines were transfected with wt-p53 cDNA or the s-myc gene linked to the 
      mouse mammary tumor virus (MMTV) promoter. Of several G418-resistant clones 
      obtained from each transfection, a few expressed the s-Myc or wt-p53 proteins. 
      Independent of mutations in the intrinsic p53 gene, the cellular growth in vitro 
      and tumorigenicity in nude mice of these clones were drastically suppressed, the 
      extent of suppression being correlated with the expression level of the 
      transfected gene. Flow-cytometric analysis demonstrated that both p53 and s-Myc 
      arrested the cell cycle at the G1/S boundary. These data suggest that these genes 
      having negative effects on tumor cell proliferation could be used in gene therapy 
      of gliomas, which are caused by alteration of the p53 gene or by some other 
      genetic change.
FAU - Asai, A
AU  - Asai A
AD  - Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
FAU - Miyagi, Y
AU  - Miyagi Y
FAU - Sugiyama, A
AU  - Sugiyama A
FAU - Gamanuma, M
AU  - Gamanuma M
FAU - Hong, S H
AU  - Hong SH
FAU - Takamoto, S
AU  - Takamoto S
FAU - Nomura, K
AU  - Nomura K
FAU - Matsutani, M
AU  - Matsutani M
FAU - Takakura, K
AU  - Takakura K
FAU - Kuchino, Y
AU  - Kuchino Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
GS  - p53
GS  - s-Myc
MH  - Animals
MH  - Base Sequence
MH  - Brain Neoplasms/*genetics/metabolism/therapy
MH  - Cell Cycle/genetics
MH  - Gene Amplification
MH  - Gene Expression
MH  - Genes, myc/*genetics/physiology
MH  - Genes, p53/*genetics/physiology
MH  - Glioma/*genetics/metabolism/therapy
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Point Mutation/*genetics
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Rats
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF01053280 [doi]
PST - ppublish
SO  - J Neurooncol. 1994;19(3):259-68. doi: 10.1007/BF01053280.