PMID- 7813363
OWN - NLM
STAT- MEDLINE
DCOM- 19950208
LR  - 20190909
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 11
IP  - 2
DP  - 1994
TI  - Comparative study of interphase cytogenetics, flow cytometric analysis, and 
      nuclear grade of fine-needle aspirates of breast carcinoma.
PG  - 151-8
AB  - The correlation between DNA ploidy and S-phase fraction (SPF) by flow cytometry 
      (FCM) and the detection of numerical chromosomal abnormalities (NCA) by 
      interphase cytogenetics (IC) involving chromosomes 8 and 12 was studied in 20 
      human breast lesions (17 breast carcinomas, 2 fibroadenomas, and 1 sclerosing 
      adenosis). Initial diagnosis was performed on fine-needle aspiration biopsy 
      (FNAB) material with subsequent histologic confirmation. FCM was performed on 
      formalin-fixed paraffin-embedded tissue while IC by fluorescence in situ 
      hybridization (FISH) was done on alcohol fixed FNAB materials. Sixteen (80%) 
      cases showed concordance between FCM and IC with respect to the presence or 
      absence of aneuploidy. The remainder of the cases (20%), which were all malignant 
      neoplasms, showed discrepancies between the two methods, all four were 
      DNA-diploid with low SPF by FCM but showed various NCA by IC. Nuclear grades (NG) 
      of all the malignant samples were also evaluated and correlated with both FCM and 
      IC studies. Although a good correlation was observed between NG and FCM, a better 
      correlation was seen between NG and IC. This study shows that although IC by FISH 
      correlated well with FCM analysis, it can detect NCA in DNA-diploid, low SPF 
      tumors. It also correlates well with the NG of the tumor. The increased 
      sensitivity provided by IC in detecting aneuploidy may be of great prognostic 
      significance in low stage, DNA-diploid, low SPF breast carcinomas.
FAU - Cajulis, R S
AU  - Cajulis RS
AD  - Department of Pathology, Northwestern University Medical School, Chicago, IL.
FAU - Kotliar, S
AU  - Kotliar S
FAU - Haines, G K
AU  - Haines GK
FAU - Frias-Hidvegi, D
AU  - Frias-Hidvegi D
FAU - O'Gorman, M
AU  - O'Gorman M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/ultrastructure
MH  - Cell Nucleus/*pathology
MH  - Cytogenetics/*methods
MH  - DNA, Neoplasm/genetics
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Ploidies
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/dc.2840110209 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 1994;11(2):151-8. doi: 10.1002/dc.2840110209.