PMID- 7813420
OWN - NLM
STAT- MEDLINE
DCOM- 19950203
LR  - 20240109
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 13
IP  - 24
DP  - 1994 Dec 15
TI  - Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin 
      biosynthesis.
PG  - 5818-25
AB  - Several genes critical to the enzymatic regulation of melanin production in 
      mammals have recently been cloned and mapped to the albino, brown and slaty loci 
      in mice. All three genes encode proteins with similar structures and features, 
      but with distinct catalytic capacities; the functions of two of those gene 
      products have previously been identified. The albino locus encodes tyrosinase, an 
      enzyme with three distinct melanogenic functions, while the slaty locus encodes 
      tyrosinase-related protein 2 (TRP2), an enzyme with a single specific, but 
      distinct, function as DOPAchrome tautomerase. Although the brown locus, encoding 
      TRP1, was actually the first member of the tyrosinase gene family to be cloned, 
      its catalytic function (which results in the production of black rather than 
      brown melanin) has been in general dispute. In this study we have used two 
      different techniques (expression of TRP1 in transfected fibroblasts and 
      immunoaffinity purification of TRP1 from melanocytes) to examine the enzymatic 
      function(s) of TRP1. The data demonstrate that the specific melanogenic function 
      of TRP1 is the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) to a 
      carboxylated indole-quinone at a down-stream point in the melanin biosynthetic 
      pathway. This enzyme activity appears to be essential to the further metabolism 
      of DHICA to a high molecular weight pigmented biopolymer.
FAU - Kobayashi, T
AU  - Kobayashi T
AD  - Laboratory of Cell Biology, National Cancer Institute, National Institutes of 
      Health, Bethesda, MD 20892.
FAU - Urabe, K
AU  - Urabe K
FAU - Winder, A
AU  - Winder A
FAU - Jimenez-Cervantes, C
AU  - Jimenez-Cervantes C
FAU - Imokawa, G
AU  - Imokawa G
FAU - Brewington, T
AU  - Brewington T
FAU - Solano, F
AU  - Solano F
FAU - Garcia-Borron, J C
AU  - Garcia-Borron JC
FAU - Hearing, V J
AU  - Hearing VJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (Tyrp1 protein, mouse)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - Z3OC8499KG (5,6-dihydroxyindole)
SB  - IM
MH  - Animals
MH  - Cell-Free System
MH  - Chromatography, Affinity
MH  - Indoles/*metabolism
MH  - Melanins/*biosynthesis
MH  - Melanocytes/metabolism
MH  - Melanoma/metabolism
MH  - *Membrane Glycoproteins
MH  - Mice
MH  - *Monophenol Monooxygenase
MH  - Oxidation-Reduction
MH  - *Oxidoreductases
MH  - Proteins/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC395555
EDAT- 1994/12/15 00:00
MHDA- 1994/12/15 00:01
PMCR- 1995/12/15
CRDT- 1994/12/15 00:00
PHST- 1994/12/15 00:00 [pubmed]
PHST- 1994/12/15 00:01 [medline]
PHST- 1994/12/15 00:00 [entrez]
PHST- 1995/12/15 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1994.tb06925.x [doi]
PST - ppublish
SO  - EMBO J. 1994 Dec 15;13(24):5818-25. doi: 10.1002/j.1460-2075.1994.tb06925.x.