PMID- 7813786
OWN - NLM
STAT- MEDLINE
DCOM- 19950209
LR  - 20131121
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 166
IP  - 2
DP  - 1994 Dec
TI  - Activation of Xenopus MyoD transcription by members of the MEF2 protein family.
PG  - 683-95
AB  - Members of the MEF2 family of DNA binding proteins interact with a set of AT-rich 
      sequences commonly found in the promoters and enhancers of muscle-specific genes. 
      We have shown that a MEF2 binding site precisely overlaps the TFIID binding site 
      (TATA box) in the Xenopus MyoDa (XMyoDa) promoter and appears to play an 
      important role in muscle-specific activity of this promoter. To further 
      investigate the potential role of MEF2 in the regulation of XMyoDa transcription, 
      we have analyzed the appearance of factors that interact with the XMyoDa 
      TATA/MEF2 site during early amphibian development. Proteins that bind 
      specifically to this site were present at low levels during early development and 
      increased in abundance during gastrulation and neurulation. Two related cDNAs 
      were isolated that encode proteins that recognize the XMyoDa TATA motif. Both 
      proteins are highly homologous to each other, belong to the MADS (MCM1 agamous 
      deficiens SRF) protein family, and are most highly related to the mammalian MEF2A 
      gene products. Xenopus MEF2A (XMEF2A) transcripts accumulated preferentially in 
      forming somites after the appearance of XMyoD transcripts. Ectopic expression of 
      XMEF2A and other members of the MEF2 gene family activated transcription of a 
      reporter gene controlled by the XMyoDa promoter. Transcriptional activation of 
      the XMyoDa promoter required only the conserved DNA binding domain of XMEF2A and 
      was independent of a domain necessary for activity when this factor was bound to 
      multiple upstream sites. These results suggest that the XMyoDa promoter can be 
      activated by binding of MEF2 to the XMyoDa TATA motif and indicate that 
      MEF2-dependent transcriptional activation occurs by different mechanisms 
      depending on the location of the MEF2 binding site. We suggest that XMEF2 
      expression in myogenic cells contributes to the activation and stabilization of 
      XMyoDa transcription during muscle cell differentiation.
FAU - Wong, M W
AU  - Wong MW
AD  - Department of Biochemistry and Molecular Biology, University of Texas M.D. 
      Anderson Cancer Center, Houston 77030.
FAU - Pisegna, M
AU  - Pisegna M
FAU - Lu, M F
AU  - Lu MF
FAU - Leibham, D
AU  - Leibham D
FAU - Perry, M
AU  - Perry M
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MEF2 Transcription Factors)
RN  - 0 (MyoD Protein)
RN  - 0 (Myogenic Regulatory Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - DNA Primers/chemistry
MH  - DNA-Binding Proteins/*genetics/physiology
MH  - Gastrula
MH  - Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - MEF2 Transcription Factors
MH  - Molecular Sequence Data
MH  - MyoD Protein/*genetics
MH  - Myogenic Regulatory Factors
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - TATA Box
MH  - Transcription Factors/*genetics
MH  - Transcription, Genetic
MH  - Xenopus laevis/*embryology/genetics
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - S0012-1606(84)71347-9 [pii]
AID - 10.1006/dbio.1994.1347 [doi]
PST - ppublish
SO  - Dev Biol. 1994 Dec;166(2):683-95. doi: 10.1006/dbio.1994.1347.