PMID- 7814479
OWN - NLM
STAT- MEDLINE
DCOM- 19950209
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 32
IP  - 10
DP  - 1994 Oct
TI  - Identification of human herpesvirus 6 variants A and B by amplimer hybridization 
      with variant-specific oligonucleotides and amplification with variant-specific 
      primers.
PG  - 2434-40
AB  - Two distinct PCR-based procedures were evaluated for the detection and 
      identification of human herpesvirus 6 (HHV-6) variants A and B in uncultured 
      human samples. Variant-specific oligonucleotide hybridization (VSOH) is based on 
      the amplification of two distinct regions of the HHV-6 genome, followed by 
      hybridization of amplimers with variant-specific oligonucleotide probes. 
      Variant-specific primer PCR (VSPP) is based on the amplification of each variant 
      by using variant-specific primers. The study of 10 well-characterized HHV-6 
      strains allowed us to demonstrate the high sensitivity and specificity of both 
      methods. With variant mixtures, however, some limitations of VSOH were evidenced 
      and VSPP was required to obtain unambiguous results. The combination of VSOH and 
      VSPP was applied to the direct study of 300 peripheral blood mononuclear cell 
      samples from French subjects. HHV-6 was detected in 15 samples: 11 corresponded 
      to variant B, 3 corresponded to variant A, and 1 corresponded to a mixture of 
      both variants.
FAU - Aubin, J T
AU  - Aubin JT
AD  - Laboratoire de Virologie-CNRS EP 57, C.E.R.V.I., Hopital de la Pitie-Salpetriere, 
      Paris, France.
FAU - Poirel, L
AU  - Poirel L
FAU - Robert, C
AU  - Robert C
FAU - Huraux, J M
AU  - Huraux JM
FAU - Agut, H
AU  - Agut H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Child, Preschool
MH  - DNA, Viral/analysis
MH  - Herpesvirus 6, Human/*classification/genetics
MH  - Humans
MH  - Infant
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - *Nucleic Acid Hybridization
MH  - Oligonucleotides/genetics
MH  - *Polymerase Chain Reaction
MH  - Sensitivity and Specificity
PMC - PMC264080
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
PMCR- 1994/10/01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.32.10.2434-2440.1994 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1994 Oct;32(10):2434-40. doi: 10.1128/jcm.32.10.2434-2440.1994.