PMID- 7815028
OWN - NLM
STAT- MEDLINE
DCOM- 19950203
LR  - 20151119
IS  - 0022-1198 (Print)
IS  - 0022-1198 (Linking)
VI  - 39
IP  - 6
DP  - 1994 Nov
TI  - 125I radioimmunoassay for the dual detection of amphetamine and methamphetamine.
PG  - 1486-96
AB  - A radioimmunoassay that exhibits a nearly equivalent response to D-amphetamine 
      and D-methamphetamine in urine over the assay range of 0 to 1000 ng/mL while 
      displaying low cross-reactivity to L-amphetamine and L-methamphetamine (4.6% and 
      2.4%, respectively) has been developed. In addition, methylenedioxy-amphetamine 
      (MDA) and methylenedioxymethamphetamine (MDMA) were detectable in the assay with 
      cross-reactivity levels of > 100% and 77% respectively. Little cross-reactivity 
      was observed with the commonly encountered over-the-counter (OTC) drugs and this 
      cross-reactivity was further reduced by the addition of sodium periodate into the 
      reaction mixture to oxidize the beta-hydroxylamines. The double (second) antibody 
      assay uses 125I-radiolabeled derivatives of both D-amphetamine and 
      D-methamphetamine as tracers in combination with two highly specific sheep 
      antisera directed against D-amphetamine and D-methamphetamine. The assay exhibits 
      a dose-response of approximately 90,000 dpm from 0 to 1000 ng/mL of D-amphetamine 
      or D-methamphetamine with a minimum detectable dose for either drug of 
      approximately 25 ng/mL. With a cut-off level of 500 ng/mL, the assay gave a 
      positive result for 100% of the 111 clinical samples containing GC/MS confirmed 
      (at or above the NIDA GC/MS cut-off values) levels of amphetamine and/or 
      methamphetamine. Eighty-eight samples that screened negative in a clinical 
      laboratory were all negative in the assay. Nineteen samples which were 
      incorrectly identified as positive by other commercially available amphetamine 
      assays were negative in this RIA.
FAU - Ward, C
AU  - Ward C
AD  - Department of Toxicology, Roche Diagnostic Systems, Inc., Branchburg, NJ.
FAU - McNally, A J
AU  - McNally AJ
FAU - Rusyniak, D
AU  - Rusyniak D
FAU - Salamone, S J
AU  - Salamone SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (Iodine Radioisotopes)
RN  - 44RAL3456C (Methamphetamine)
RN  - CK833KGX7E (Amphetamine)
SB  - IM
MH  - Amphetamine/*analysis
MH  - Calibration
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Iodine Radioisotopes
MH  - Methamphetamine/*analysis
MH  - Predictive Value of Tests
MH  - Radioimmunoassay/*methods
MH  - Sensitivity and Specificity
MH  - Substance Abuse Detection/*methods
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
PST - ppublish
SO  - J Forensic Sci. 1994 Nov;39(6):1486-96.