PMID- 7816810
OWN - NLM
STAT- MEDLINE
DCOM- 19950209
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 1
DP  - 1995 Jan 3
TI  - Optical mapping of site-directed cleavages on single DNA molecules by the 
      RecA-assisted restriction endonuclease technique.
PG  - 165-9
AB  - Fluorescence in situ hybridization (FISH) resolution has advanced because newer 
      techniques use increasingly decondensed chromatin. FISH cannot analyze 
      restriction enzyme cutting sites due to limitations of the hybridization and 
      detection technologies. The RecA-assisted restriction endonuclease (RARE) 
      technique cleaves chromosomal DNA at a single EcoRI site within a given gene or 
      selected sequence. We recently described a mapping technique, optical mapping, 
      which uses fluorescence microscopy to produce high-resolution restriction maps 
      rapidly by directly imaging restriction digestion cleavage events occurring on 
      single deproteinized DNA molecules. Ordered maps are then constructed by noting 
      fragment order and size, using several optically based techniques. Since we also 
      wanted to map arbitrary sequences and gene locations, we combined RARE with 
      optical mapping to produce site-specific visible EcoRI restriction cleavage sites 
      on single DNA molecules. Here we describe this combined method, named optical 
      RARE, and its initial application to mapping gene locations on yeast chromosomes.
FAU - Wang, Y K
AU  - Wang YK
AD  - W.M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York 
      University, New York 10003.
FAU - Huff, E J
AU  - Huff EJ
FAU - Schwartz, D C
AU  - Schwartz DC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Fungal)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - EC 2.7.7.- (Rec A Recombinases)
RN  - EC 3.1.21.- (Deoxyribonuclease EcoRI)
SB  - IM
GS  - ERG16
GS  - LEU2
GS  - THR4
MH  - Base Sequence
MH  - Binding Sites
MH  - Candida albicans/*genetics
MH  - Chromosomes, Fungal
MH  - DNA, Fungal/*chemistry/*metabolism
MH  - Deoxyribonuclease EcoRI
MH  - *Genes, Fungal
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Microscopy, Fluorescence/methods
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - *Rec A Recombinases/metabolism
MH  - *Restriction Mapping
MH  - Saccharomyces cerevisiae/*genetics
PMC - PMC42838
EDAT- 1995/01/03 00:00
MHDA- 1995/01/03 00:01
PMCR- 1995/07/03
CRDT- 1995/01/03 00:00
PHST- 1995/01/03 00:00 [pubmed]
PHST- 1995/01/03 00:01 [medline]
PHST- 1995/01/03 00:00 [entrez]
PHST- 1995/07/03 00:00 [pmc-release]
AID - 10.1073/pnas.92.1.165 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):165-9. doi: 10.1073/pnas.92.1.165.