PMID- 7817130
OWN - NLM
STAT- MEDLINE
DCOM- 19950208
LR  - 20170214
IS  - 0192-6233 (Print)
IS  - 0192-6233 (Linking)
VI  - 22
IP  - 4
DP  - 1994 Jul-Aug
TI  - Enzymic pattern of preneoplastic and neoplastic lesions induced in the kidney of 
      CBA mice by 1,2-dimethylhydrazine.
PG  - 415-22
AB  - Mouse renal cell tumors (RCTs) were induced in male CBA mice by 5 subcutaneous 
      injections of 8 mg 1,2-dimethylhydrazine (DMH)/kg body weight once a week. After 
      a lag period of 2 yr kidneys were removed, and serial cryostat sections of the 
      kidneys were histochemically analyzed for the following parameters: glycogen 
      content, basophilia, and the activities of glycogen synthase (SYN), glycogen 
      phosphorylase (PHO), glucose-6-phosphatase (G6Pase), glucose-6-phosphate 
      dehydrogenase (G6PDH), hexokinase (HK), pyruvate kinase (PK), lactate 
      dehydrogenase (LDH), malic enzyme (ME), succinate dehydrogenase (SDH), alkaline 
      phosphatase (ALPase) and gamma-glutamyltranspeptidase (GGT). RCTs displayed the 
      same histochemical profile irrespective of their size and growth pattern. In 
      comparison with the normal kidney epithelium, the neoplastic cells exhibited 
      elevated activities of enzymes for glycolysis (HK, PK, LDH) and the pentose 
      phosphate pathway (G6PDH), while negative G6Pase and low SDH activity were 
      observed in these cells. The majority of RCTs showed high PHO activity and weak 
      staining for SYN. Activities of ALPase and GGT were negative in most of the RCTs. 
      Markedly enlarged cells with atypical nuclei were detected in some advanced RCTs. 
      Higher activities of glycolytic and mitochondrial enzymes and G6PDH were found in 
      these enlarged cells than in other tumor cells. Tubular preneoplastic lesions 
      were similar to neoplastic lesions in morphological and histochemical 
      characteristics. The present study revealed that a markedly elevated capacity for 
      glycolysis and the pentose phosphate pathway occurred in RCTs in mice. A similar 
      histochemical pattern in the few preneoplastic tubular lesions observed suggests 
      that these metabolic aberrations emerge early during carcinogenesis, but 
      additional studies on early stages of renal carcinogenesis are needed to 
      substantiate this assumption.
FAU - Ahn, Y S
AU  - Ahn YS
AD  - Abteilung fur Cytopathologie (0310), Deutsches Krebsforschungszentrum, 
      Heidelberg, Germany.
FAU - Chemeris, G Y
AU  - Chemeris GY
FAU - Turusov, V S
AU  - Turusov VS
FAU - Bannasch, P
AU  - Bannasch P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Toxicol Pathol
JT  - Toxicologic pathology
JID - 7905907
RN  - 0 (Carcinogens)
RN  - 0 (Dimethylhydrazines)
RN  - IX068S9745 (1,2-Dimethylhydrazine)
SB  - IM
MH  - 1,2-Dimethylhydrazine
MH  - Animals
MH  - Carcinogens
MH  - Dimethylhydrazines
MH  - Histocytochemistry
MH  - Kidney Neoplasms/chemically induced/*enzymology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Precancerous Conditions/chemically induced/*enzymology/pathology
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1177/019262339402200407 [doi]
PST - ppublish
SO  - Toxicol Pathol. 1994 Jul-Aug;22(4):415-22. doi: 10.1177/019262339402200407.