PMID- 7819102
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 88
IP  - 4
DP  - 1994 Dec
TI  - Metaphase fluorescence in situ hybridization (FISH) in the follow-up of 60 
      patients with haemopoietic malignancies.
PG  - 778-83
AB  - Metaphase DNA fluorescence in situ hybridization (metaphase-FISH) was performed 
      on follow-up samples from 60 patients suffering from haemopoietic malignancies 
      (acute and chronic myeloid leukaemia, acute lymphoblastic leukaemia, 
      non-Hodgkin's lymphoma and myelodysplastic syndrome). All patients had clonal 
      chromosomal trisomies or translocations at diagnosis, and were treated by bone 
      marrow transplantation (BMT), chemotherapy (CT) or interferon-alpha therapy. 
      Metaphase-FISH was performed during therapy-induced complete haematological 
      remission (CR) (BMT and CT patients) using biotin-labelled whole chromosome paint 
      probes. 28% of all patients in CR were shown by FISH to have abnormal metaphase 
      cells, and 62% of this group suffered a clinical relapse. Of those with negative 
      FISH results (72%), 12% relapsed. In three CML patients treated with BMT a small 
      population of t(9;22)-positive cells was demonstrated. These cells disappeared 
      during follow-up without causing a relapse. One ALL patient had abnormal cells a 
      short time after start of therapy but was also later found FISH-negative. 
      Furthermore, we demonstrated that metaphase-FISH is a suitable method for 
      quantifying the proportion of abnormal cells in CML patients during 
      interferon-alpha therapy. Metaphase-FISH was also employed to detect a local 
      relapse in an ALL patient. Thus, metaphase-FISH was found reliable and sensitive 
      for detection of minimal residual disease in patients with haemopoietic 
      malignancies.
FAU - Nylund, S J
AU  - Nylund SJ
AD  - Department of Medical Genetics, University of Helsinki, Finland.
FAU - Ruutu, T
AU  - Ruutu T
FAU - Saarinen, U
AU  - Saarinen U
FAU - Knuutila, S
AU  - Knuutila S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anemia, Refractory/*genetics/therapy
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia/*genetics/therapy
MH  - Lymphoma, Non-Hodgkin/*genetics/therapy
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Neoplasm, Residual
MH  - Remission Induction
MH  - Translocation, Genetic
MH  - Trisomy
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1994.tb05117.x [doi]
PST - ppublish
SO  - Br J Haematol. 1994 Dec;88(4):778-83. doi: 10.1111/j.1365-2141.1994.tb05117.x.