PMID- 7819130
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20091119
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 5
IP  - 9
DP  - 1994 Sep
TI  - Characterization of platelet-derived growth factor alpha receptor synthesis and 
      metabolic turnover.
PG  - 937-42
AB  - Cellular responses to the AA isoform of platelet-derived growth factor (PDGF-AA) 
      are mediated via the PDGF alpha receptor. Several studies suggest this receptor 
      may signal pathways distinct from those activated by the PDGF beta receptor. 
      Because alpha receptors are less well characterized than are beta receptors, and 
      because the quantity of cell surface PDGF receptors governs the extent and 
      perhaps type of PDGF-stimulated response, we examined the synthesis and 
      degradation of alpha receptors in BALB/c-3T3 cells. Our data show that the 
      ligand-independent half-life of alpha receptors is 3 h and that optimal turnover 
      of alpha receptors requires protein synthesis. In the presence of ligand, the 
      half-life of alpha receptors markedly decreases and is independent of protein 
      synthesis. Although PDGF-AA accelerated the rate of alpha receptor turnover, 
      pretreatment of cells with PDGF-AA and essentially complete down-regulation of 
      alpha receptors did not correspondingly increase the level of alpha receptor 
      synthesis. These findings indicate that the number of cell surface PDGF alpha 
      receptors is regulated by the rate of internalization of these receptors. Lastly, 
      we report that the recovery of PDGF-AA binding following down-regulation of alpha 
      receptors is not affected by inhibition of RNA synthesis. Thus, repopulation of 
      cell surface PDGF alpha receptors may not necessitate an increase in the level of 
      PDGF alpha receptor mRNA.
FAU - Coats, S R
AU  - Coats SR
AD  - Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, 
      Tennessee 37232-0615.
FAU - Olashaw, N E
AU  - Olashaw NE
FAU - Pledger, W J
AU  - Pledger WJ
LA  - eng
GR  - CA09385/CA/NCI NIH HHS/United States
GR  - CA09502/CA/NCI NIH HHS/United States
GR  - CA43720/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 0 (Ligands)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (platelet-derived growth factor A)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Down-Regulation/physiology
MH  - Half-Life
MH  - Ligands
MH  - Mice
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Receptor, Platelet-Derived Growth Factor alpha
MH  - Receptors, Platelet-Derived Growth Factor/biosynthesis/*metabolism
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1994 Sep;5(9):937-42.