PMID- 7821088
OWN - NLM
STAT- MEDLINE
DCOM- 19950215
LR  - 20190830
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 103
IP  - 5
DP  - 1994 Sep
TI  - Physical mapping of 5S rDNA reveals a new locus on 3R and unexpected complexity 
      in a rye translocation used in chromosome mapping.
PG  - 331-7
AB  - Using fluorescence in situ hybridization (FISH) with probe pScT7, three different 
      5S rDNA loci were detected in the satellite of rye chromosome 1R (5SDna-R1) and 
      in the short arms of chromosomes 3R (5SDna-R3) and 5R (5SDna-R2) respectively. 
      All three loci showed polymorphism for the hybridization signal intensity. In 
      order to determine the localization of these rye 5S rDNA multigene loci with 
      higher precision within the corresponding chromosome arms, the probe pScT7 was 
      physically mapped by FISH in relation to the following five translocations 
      (Wageningen Tester Set): T850W (1RS/4RL), T248W (1RS/6RS), T273W (1RS/5RL), T305W 
      (2RS/5RS) and T240W (3RS/5RL). Accurate physical maps of the translocation 
      breakpoints had previously been made using electron microscope analysis of spread 
      pachytene synaptonemal complexes of heterozygotes for the different 
      translocations. The results indicate that locus 5SDna-R3 is located between the 
      breakpoint of translocation T240W and the telomere, whereas locus 5SDna-R2 is 
      located between the breakpoint of translocation T305W and the centromere, the 
      hybridization of probe pScT7 on T305W translocated chromosomes demonstrating the 
      complex nature of this translocation. On the other hand, the simultaneous 
      detection of probes pScT7 and pTA71 (18S-5.8S-26S rDNA) with two different 
      fluorochromes, indicated that the breakpoints of translocations T850W and T248W 
      are located between loci Nor-R1 and 5SDna-R1.
FAU - Alonso-Blanco, C
AU  - Alonso-Blanco C
AD  - Departamento de Biologia Funcional, Universidad de Oviedo, Spain.
FAU - Pendas, A M
AU  - Pendas AM
FAU - Garcia-Suarez, R
AU  - Garcia-Suarez R
FAU - Roca, A
AU  - Roca A
FAU - Goicoechea, P G
AU  - Goicoechea PG
FAU - Giraldez, R
AU  - Giraldez R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - *Chromosome Mapping
MH  - DNA Probes
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Genetic
MH  - RNA, Plant/genetics
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Secale/*genetics
MH  - Translocation, Genetic/*genetics
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1007/BF00417880 [doi]
PST - ppublish
SO  - Chromosoma. 1994 Sep;103(5):331-7. doi: 10.1007/BF00417880.