PMID- 7821809 OWN - NLM STAT- MEDLINE DCOM- 19950214 LR - 20190707 IS - 0378-1119 (Print) IS - 0378-1119 (Linking) VI - 150 IP - 2 DP - 1994 Dec 15 TI - Sequence and chromosomal localisation of the gene encoding ovine latent transforming growth factor-beta 1. PG - 371-3 AB - Transforming growth factor-beta 1 (TGF-beta 1) plays important roles in pathologic processes. To further investigate the actions of this cytokine in sheep, the entire 1170-bp ovine TGF-beta 1 pro-protein-encoding sequence has been determined by the cloning and sequencing of specific polymerase-chain-reaction amplification products of TGF-beta 1 cDNA sequences. In addition, these sequences have been used to estimate the length of the TGF-beta 1 mRNA as 1.5-1.7 kb by Northern blot hybridization and determine that the ovine TGF-beta 1 gene occupies a single locus in the sheep genome by chromosomal in situ hybridization. FAU - Woodall, C J AU - Woodall CJ AD - Department of Veterinary Pathology, Summerhall, University of Edinburgh, UK. FAU - McLaren, L J AU - McLaren LJ FAU - Watt, N J AU - Watt NJ LA - eng SI - GENBANK/X76916 PT - Journal Article PL - Netherlands TA - Gene JT - Gene JID - 7706761 RN - 0 (DNA, Complementary) RN - 0 (RNA, Messenger) RN - 0 (Transforming Growth Factor beta) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Blotting, Northern MH - Chromosome Mapping MH - Cloning, Molecular MH - DNA, Complementary MH - Molecular Sequence Data MH - Open Reading Frames MH - Polymerase Chain Reaction MH - RNA, Messenger/biosynthesis MH - Sheep/*genetics MH - Transforming Growth Factor beta/biosynthesis/*genetics EDAT- 1994/12/15 00:00 MHDA- 1994/12/15 00:01 CRDT- 1994/12/15 00:00 PHST- 1994/12/15 00:00 [pubmed] PHST- 1994/12/15 00:01 [medline] PHST- 1994/12/15 00:00 [entrez] AID - 0378-1119(94)90455-3 [pii] AID - 10.1016/0378-1119(94)90455-3 [doi] PST - ppublish SO - Gene. 1994 Dec 15;150(2):371-3. doi: 10.1016/0378-1119(94)90455-3.