PMID- 7821960
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 83
IP  - 1
DP  - 1994 Sep
TI  - The type of interaction with Fc gamma R in human monocytes determines the 
      efficiency of the generation of oxidative burst.
PG  - 148-54
AB  - Receptors for the Fc fragment of IgG (Fc gamma R) have a well-documented role in 
      the generation of oxidative burst. It is tempting to speculate that the type of 
      interaction with Fc gamma R could be a mechanism of regulation of this process. 
      Here we report on a comparative study of the induction of oxidative burst in 
      human monocytes activated by means of different types of interaction with Fc 
      gamma R. We studied non-primed monocytes obtained by centrifugal elutriation from 
      healthy donors. These cells were submitted to Fc gamma R interactions following 
      two distinct models: one, using particulate material (IgG-SRBC leading to 
      phagocytosis or rosetting), and another using soluble reagents followed by 
      cross-linking of the receptors (monoclonal antibodies against Fc gamma RI and Fc 
      gamma RII and natural ligands, namely several isotypes of murine and human IgG). 
      Phagocytosis and oxidative burst were studied simultaneously in the monocytes, 
      following the methodology described recently. Human non-primed monocytes were 
      able to generate a very obvious oxidative burst response after activation of Fc 
      gamma R by particulate material. The same response was observed when Fc gamma RII 
      was blocked by monoclonal antibodies. Ingestion was not necessary for activation 
      of the oxidative burst, since the model of rosetting induced a level of burst 
      generation similar to the one obtained in the phagocytic process. Cross-linking 
      of Fc gamma RI by soluble reagents induced production of reactive oxidative 
      intermediates (ROI) only when the ligand-binding site of the receptor was 
      involved. These data lead to the conclusion that Fc gamma R interaction with 
      soluble or particulate material induces oxidative burst in non-primed human 
      monocytes only when the binding site of natural ligands is involved. The type of 
      interaction also determines the efficiency of the generation of ROI. This fact 
      could represent a regulatory mechanism.
FAU - Casado, J A
AU  - Casado JA
AD  - Department of Immunology, Faculty of Medicine, University of Navarra, Pamplona, 
      Spain.
FAU - Merino, J
AU  - Merino J
FAU - Cid, J
AU  - Cid J
FAU - Subira, M L
AU  - Subira ML
FAU - Sanchez-Ibarrola, A
AU  - Sanchez-Ibarrola A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Cross-Linking Reagents/pharmacology
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Monocytes/immunology/*metabolism
MH  - Phagocytosis/physiology
MH  - Receptors, IgG/immunology/*metabolism
MH  - Respiratory Burst/drug effects/*physiology
MH  - Rosette Formation
PMC - PMC1415001
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
PMCR- 1995/09/01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PHST- 1995/09/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1994 Sep;83(1):148-54.