PMID- 7822413
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 128
IP  - 1-2
DP  - 1995 Jan
TI  - Roles of hepatocyte growth factor/scatter factor and the met receptor in the 
      early development of the metanephros.
PG  - 171-84
AB  - Several lines of evidence suggest that hepatocyte growth factor/scatter factor 
      (HGF/SF), a soluble protein secreted by embryo fibroblasts and several fibroblast 
      lines, may elicit morphogenesis in adjacent epithelial cells. We investigated the 
      role of HGF/SF and its membrane receptor, the product of the c-met protooncogene, 
      in the early development of the metanephric kidney. At the inception of the mouse 
      metanephros at embryonic day 11, HGF/SF was expressed in the mesenchyme, while 
      met was expressed in both the ureteric bud and the mesenchyme, as assessed by 
      reverse transcription PCR, in situ hybridization, and immunohistochemistry. To 
      further investigate the expression of met in renal mesenchyme, we isolated 13 
      conditionally immortal clonal cell lines from transgenic mice expressing a 
      temperature-sensitive mutant of the SV-40 large T antigen. Five had the 
      HGF/SF+/met+ phenotype and eight had the HGF/SF-/met+ phenotype. None had the 
      HGF/SF+/met- nor the HGF/SF-/met- phenotypes. Thus the renal mesenchyme contains 
      cells that express HGF/SF and met or met alone. When metanephric rudiments were 
      grown in serum-free organ culture, anti-HGF/SF antibodies (a) inhibited the 
      differentiation of metanephric mesenchymal cells into the epithelial precursors 
      of the nephron; (b) increased cell death within the renal mesenchyme; and (c) 
      perturbed branching morphogenesis of the ureteric bud. These data provide the 
      first demonstration for coexpression of the HGF/SF and met genes in mesenchymal 
      cells during embryonic development and also imply an autocrine and/or paracrine 
      role for HGF/SF and met in the survival of the renal mesenchyme and in the 
      mesenchymal-epithelial transition that occurs during nephrogenesis. They also 
      confirm the postulated paracrine role of HGF/SF in the branching of the ureteric 
      bud.
FAU - Woolf, A S
AU  - Woolf AS
AD  - Units of Developmental Biology and Medicine, Institute of Child Health, London, 
      United Kingdom.
FAU - Kolatsi-Joannou, M
AU  - Kolatsi-Joannou M
FAU - Hardman, P
AU  - Hardman P
FAU - Andermarcher, E
AU  - Andermarcher E
FAU - Moorby, C
AU  - Moorby C
FAU - Fine, L G
AU  - Fine LG
FAU - Jat, P S
AU  - Jat PS
FAU - Noble, M D
AU  - Noble MD
FAU - Gherardi, E
AU  - Gherardi E
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Antibodies)
RN  - 0 (DNA Primers)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
GS  - c-met
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Base Sequence
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - DNA Primers
MH  - Embryonic and Fetal Development
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/analysis/*biosynthesis
MH  - Interferon-gamma/pharmacology
MH  - Kidney/cytology/*embryology/*metabolism
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Mice, Transgenic
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Organ Culture Techniques
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-met
MH  - Proto-Oncogenes
MH  - Receptor Protein-Tyrosine Kinases/*biosynthesis
MH  - Time Factors
PMC - PMC2120323
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 1995/07/01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 1995/07/01 00:00 [pmc-release]
AID - 95122630 [pii]
AID - 10.1083/jcb.128.1.171 [doi]
PST - ppublish
SO  - J Cell Biol. 1995 Jan;128(1-2):171-84. doi: 10.1083/jcb.128.1.171.